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《Chinese Journal of Veterinary Medicine》 2014-09
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An ELISA method to detect the residue of avermectins in bovine tissues

YANG Jun-hong;HE Ji-hong;HOU Xiao-lin;QI Peng;WU Jia-xin;China Animal Husbandry Group;China Animal Husbandry Industry CO. LTD;Beijing University of Agriculture;  
An ELISA method was developed to detect AVM,IVM,and EPR residues in bovine tissues. The formula was y =-45.613 x + 81.191,R2= 0.9951,and the 50% inhibition concentration(IC50) was 4.8 ng/mL. The linear detecting range of calibrationcurve was from 1.1 ng/mL to 21.9 ng/mL. When the spiked concentrations of AVMs were 20 ng/g,50 ng/g, and 100 ng/g in bovine liv-er,the recovery of AVMs was 53.8%~80.4% with coefficients of variation(CV) of 3.4%~17.9%. When the spiked concentrations ofAVMs were 5 ng/g,10 ng/g,and 20 ng/g in bovine muscle,the recovery of AVMs was 70.9%~108.6% with CV of 3.7%~17.1%.
【Fund】: “863”国家高技术研究计划项目(2011AA10A214)
【CateGory Index】: S859.84
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