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《Journal of Traditional Chinese Veterinary Medicine》 2013-01
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Isolation and culture of Holstein-bovine mammary epithelial cells

LI Shi-wei,GU Jing-juan,ZHAO Lu,LI Long,WANG Xue-tao,KUANG Ling(Veterinary College of Xinjiang Agricultural University,Urumqi Xinjiang 830052,China)  
Objective:To establish the methods of isolation and culture of Holstein-bovine mammary epithelial cells.Methods:Tissues explant was employed to culture bovine mammary epithelial cells and trypsin digesting from time interval was used to purify the cells.Results:The mammary gland epithelial cells were cultivating.When observed by microscope,the purified cells showed typical characters of epithelial cells,tightly arranging cell to cell,flagstone-like,homogeneous cell morphology.The tissue specific expression of cytokeratin 18 gene in mammary epithelial cells was detected by immunofluorescent cytochemical staining.Bovine mammary gland epithelial cells proliferated exuberantly when cultured up to passage 25.Conclusion:Purified bovine mammary gland epithelial cells were obtained by using tissues explant culture and trypsin digesting from time interval.
【Fund】: 国家自然科学基金(30960292);; 新疆维吾尔自治区普通高校重点学科基础兽医学项目资助
【CateGory Index】: S823
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