Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Stereology and Image Analysis》 2009-01
Add to Favorite Get Latest Update

Morphometric study on the Raji cell damages induced by combination of gamma ray and microwave radiation

XIA Hongjie1,2,WANG Dewen1,ZUO Hongyan1,XU Xinping1,GAO Yabing1,PENG Ruiyun1,JIA Kai1,QIU Bingtao1(1.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China;2.The Rehabilitation Physiotherapy Center of Integrated Traditional Chinese and Western Medicine, General Hospital of Chinese Peoples Armed Police Force,Beijing 100039,China)  
Objective To investigate the apoptosis and necrosis in Raji cells induced by combined microwave and γ ray irradiation and the association with intracellular Ca2+ concentration changes.Method The cells of Raji cell line were used in this experiment and divided into 4 groups: S-HPM irradiation alone,γ ray irradiation alone,γ+S-HPM,and control groups.Annexin-V and PI double labeling were used to detect the apoptosis and necrosis in the Raji cells.Fluo-3 fluorescent probe labeling and laser scanning confocal microscopy were applied to examine the changes of intracellular free Ca2+ concentration.Results 1.Apoptosis and necrosis: γ ray combined with microwave irradiation induced apoptosis and necrosis in Raji cells.The amount of apoptotic and necrotic cells in both γ ray irradiation group and γ+S-HPM group were significantly more than those of control group.Moreover,the amount of apoptotic cells in the combined radiation group was significantly more than that in γ ray irradiation group.2.Ca2+ concentration: The Ca2+ fluorescence intensities in cells of S-HPM,γ and γ+S-HPM radiation groups were significantly stronger than that of control group,There was significant difference between any two radiation groups.The Ca2+ fluorescence intensity was in a order of S-HPMγγ+S-HPM.Conclusion Both microwave and γ ray increase apoptosis and necrosis in Raji cells.The injury effect of γ+S-HPM combined irradiation is more serious than any single irradiation on Raji cells.The major injury dipicted under conditions of this experiment is caused by γ ray irradiation,and further enhanced by microwave irradiation.Intracellular calcium overloading may be one of the injury mechanisms.
【Fund】: 国家自然科学基金(30230360)
【CateGory Index】: R818
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 JIA Yong-feng,WANG Shui-ming,WANG De-wen,et al.Department of Pathology,The Hospital Affiliated to Inner Mongolia Medical College,Hohhot 010059,China;Effect of γ-ray Combined with Microwave Radiation on Proliferation and Differentiation Potential of Bone Marrow Cells in Mice[J];Chinese Journal of Misdiagnostics;2010-15
Chinese Journal Full-text Database 8 Hits
1 JIANG Tao1, WANG De-Wen1, GAO Ya-Bing1, HU Wen-Hua1, CHEN Jian-Kui2, LIU Jie1, WANG Shui-Ming1, PENG Rui-Yun1,GU Qing-Yang1 (1.Institute of Radiation Medicine, Academy of Military Medical Sciences,Beijing 100850, China;2.Affiliated Hospital, Academy of Military Medical Sciences,Beijing 100039, China);Effect of EMP on cultured cornea epithelial cells[J];Bulletin of The Academy of Military(Medical Sciences);2004-04
2 Shui Ming WANG 1 , De Wen WANG 1 , Rui Yun PENG 1 , Ya Bing GAO 1 , Yi YANG 1 , Wen Hua HU 1 , Hao Yu CHEN 1 , You Ren ZHANG 2 , Yan GAO 1 (1.Institute of Radiation, Medicine Academy of Millitary Medical Science, Beijing 100850, China; 2. Department of Nuclear Medicine, the 307 th Hospital of PLA, Beijing 100039, China);Effect of Electromagnetic Pulse Irradiation on Structure and Function of Leydig Cells in Mice[J];Acto andrologica Sinica;2003-05
3 GUO Jing, PU Yong-mei, Donald C. Chang (ZHANG Dong-cai)(Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China);CALCIUM SIGNALING IN APOPTOSIS[J];Acta Biophysica Sinica;2005-01
4 Liu Jiang Institute of Environmental Health & Engineering, Chinese Academy of Preventive Medicine, Beijing 100050, China;Hazards of radio frequency magnetic field and their prevention and control[J];Journal of Hygiene Research;2002-05
5 CAO Xiao--zhe;WANG De--wen;ZHAO Mei--lan;ZHANC Sa Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;The atomic force microscope study on the pituitary cell membrane perforate induced by electromagnetic pulse[J];Chinese Journal of Physical Medicine and Rehabilitation;2003-08
6 YAO Li *,LIANG Xiao-li,WANG De-wen,WANG Shui-ming,SUI Xiang,ZHANG Li,GAO Ya-bing,YIN Jian-ning, PENG Rui-yun. *Department of Pathology, Beijing Medical College of PLA,Beijing 100071, China;The pathological changes in the liver of mice induced by high power electromagnetic irradiation[J];Chinese Journal of Physical Medicine and Rehabilitation;2004-02
7 LI Yu-hong, WANG De-wen, GAO Ya-bing,WANG Shui-ming, HU Wen-hua, PENG Rui-yun, LI Yang. Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing 100850, China;The injurious effects and the changes of [Ca~(2+)]_i iuduced by electromagnetic pulse on hippocampal neurons in vitro[J];Chinese Journal of Physical Medicine and Rehabilitation;2005-04
8 JIA Yongfeng,WANG Shuiming,WANG Dewen,et al(Institute of Radiation and Medicine,Academy of Military Medical Sciences,Beijing 100850,China);The morphological features of Apoptosis of bone marrow cells of mice induced by γrays irradiation combined with microwave and quantitative analysis of Bax and Bcl-2 expression[J];Chinese Journal of Stereology and Image Analysis;2006-01
Chinese Journal Full-text Database 10 Hits
1 LI Yang, SONG Liang-Wen, PENG Rui-Yun, WANG De-Wen, JIN Mei-Hong, GAO Ya-Bing, MA Jun-Jie Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing, 100850, P. R. China;Effects of SB203580 and WP631 on Smad Signal Transduction Pathway in Lung Fibroblasts after Irradiation[J];Chinese Journal of Cancer;2008-07
2 WANG Guang-lan1,CUI Jun-sheng2,WANG Xin-rui1,SHI Bo1,GAO Jing1,NI Jin-Song1(1.Key Laboratory of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China; 2.Department of Pathology,China-Japan Union Hospital,Jilin University,Changchun 130033,China);Mechanism of apoptosis of lung carcinoma cell line NCI-H460 induced by 20(S)-ginsenoside Rg3[J];Journal of Jilin University(Medicine Edition);2008-06
3 WANG Linbo,HUANG Yanlong,ZU Ling,et al.Department of Ophthalmodogy,the Tenth People's Hospital of Zhengzhou,Zhengzhou 450052,China;A Comparative Study of the Effect of Electromagnetic Pulse on Eyes of Monkey,Dog and Rabbit[J];Journal of Bethune Military Medical College;2006-03
4 LIU Shao-chen,ZHAO Xiang-yang,LI Yu-hong(Department of pathology,Chende Medical college,Hebei Chengde 067000,China);PROTECTION OF MK801 AND NIFEDIPINE FROM EMP-INDUCED DAMAGE IN HIPPOCAMPAL NEURONS IN VITRO[J];Journal of Chengde Medical College;2006-02
5 LI Jin-qing,CHEN Shao-zong,LI Xue-yong,et al. (Department of Plastics and Burns,Tangdu Hospital,FMMU,Xi′an 710038,China);Vacuum-assisted closure alleviate secondary tissue necrosis of pig′s infected soft tissue blast injury[J];Chongqing Medical Journal;2005-05
6 Gong Qianfen1,Yang Xuesen2,Li Ke3,Deng Zhaohui2,Zhang Guangbin2,Ma Hongke3,Yu Zhengping2(1Department of Nutrition,Southwest Hospital,2Department of Occupational Hygiene,College of Military Preventive Medicine,Third Military Medical University,Chongqing,400038;3Institute of Applied Electronics,Chinese Academy of Engineering Physics,Mianyang,Sichuan Province,621900,China);Protective effect of antioxidative micronutrient intervention on microwave irradiation-induced liver damage in rats[J];Acta Academiae Medicinae Militaris Tertiae;2010-02
7 ZHUANG Zhi-Qiang~1,CHEN Jing-Zao~1,PEI Zhao-Hui~2~1Department of Radiation Medicine,School of Preventive Medicine,~2Department of Physiology,School of Basic Medicine,Fourth(Military) Medical University,Xi'an 710033,China;Observation on testicular ultrastructure of rats exposed to infrasound of 8 Hz at 130 dB[J];Journal of the Fourth Military Medical University;2006-15
8 LI Jin-Qing, LI Xue-Yong, CHEN Shao-Zong, LI Wang-Zhou, LI Yue-JunDepartment of Plastics and Burns, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;Effect of infection on secondary necrosis of soft tissue blast injury in pigs[J];Journal of the Fourth Military Medical University;2007-04
9 LIN Ming-xiong,YANG Ming-hui,DOU Yong-qi,LIU Yi Department of Traditional Chinese Medicine,General Hospital of PLA,Beijing 100853,China;Liangxuehuoxue Recipe inhibits expressions of tumor necrosis factor-α and transforming growth factor-β in the lung tissue of rats with X-ray exposure[J];Journal of Southern Medical University;2011-02
10 LIU Li-juan1,ZHAO Dong-yang2,WANG Jian2,YAO Cheng-guo2,SUN Cai-xin2,TANG Jun-ying1 1Department of Gynecology and Obstetrics,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;2State Ministry of Education Key Laboratory of High Voltage and Electrical New Technology,College of Electrical Engineering,Chongqing University,Chongqing 400044,China;Ca~(2+) is an important mediator of nanosecond steep pulse-induced apoptosis in human ovarian cancer SKOV3 cells[J];Journal of Southern Medical University;2011-05
China Proceedings of conference Full-text Database 7 Hits
1 ;防电磁波辐射织物的发展与研究[A];[C];2011
2 ChenJuan, ZuoHong-yan.Wang De-Wen, Wang shui-ming, Peng Rui-yun (Department of experimental pathology, Beijing Institute of radiation medicine. 100850 Beijing, China);Morpnometry on The Injury Effects of Three Kinds of Band Electromagnetic Radiations on Hippocampus and The Expression of Injury-Related Proteins in Wistar Rats[A];[C];2006
3 Hang Wang, Xuetao Shi, Xiuzhen Dong* (Department of Biomedical Engineering, Fourth Military Medical University, Xi’an 710032);Simulation Study on Impedance Measurement Box of in Vitro Tissue Based on Comsol Finite Element Analysis[A];[C];2010
4 WU Xiao-dan~1,JIN Zhe-xiong~2,WAN Chun-lei~2,JIN Rui~2 (1 Harbin Normal University,Harbin 150025,China;2 Harbin University of Commerce,Harbin 150076, China);Hepatocellular Carcinoma HepG2 Cell Apoptosis Induced by Agrimony[A];[C];2010
5 LIU Zhi-ang CAO Bu-qing WAN Hai-zhen,etal.(Laboratory,the 303ndHospital,PLA.Nanning Guangxi 530021,China);Serum Biochemical Indexes in Human Irradiated by Microwave Radiation[A];[C];2005
6 Yu Lei1,2,3 Mo Ke2,3 Wang Wei2,3 Cui Rong-tian2,3 JI Yu-bin1,2,3 Zou Xiang1,2,3(1.Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Commerce,Harbin 150076,China;2.Institute of Materia Medica and Postdoctoral Programme of Harbin University of Commerce,Harbin 150076,China;3.Engineering Reseach Center of Natural Anticancer Drugs,Ministry of Education);Study on Preliminary Mechanism Apoptosis in HepG-2 by CSA[A];[C];2008
7 Zhang Li Wangjin Guo Guo-Zhen Department of Radiation Medicine,Faculty of preventive Medicine,The Fourth Military Medical University.Xian 710032,China;Effects of EMP exposure on the memory function of rat pups[A];[C];2007
Chinese Journal Full-text Database 3 Hits
1 Jia Yongfeng, Wang Shuiming, Chen Jiankui et al. Institute of Radiation and Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Functional and morphological changes in mouse hematopoietic system after exposure to γ-ray irradiation combined with microwave[J];Medical Journal of Chinese People's Liberation Army;2006-08
2 MENG Qian-qian,ZHOU Zhi-tian,TONG Jian,CAO Yi(Radiation Medicine and Public Health College of SuZhou University,Jiangsu Suzhou,215123,China);Combined Effects of Microwave and Ionizing Radiation on Mouse[J];Suzhou University Journal of Medical Science;2007-03
3 ZHANG Wei,CAO Yi,MENG Qian-qian,et al(College of Radiation Medicine and Public Health of Suzhou University,Suzhou 215123,China);Effect of cell growth induced by combination of microwave and γ-ray[J];China Occupational Medicine;2008-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Yi Xu Hui Ma Guirong Guo Youying;New Problems of the Electromagnetic Environment in Cities[J];Municipal Administration & Technology;2001-03
2 SHEN Shi-Ren, CHEN Jing-Zao, LIU Shcu-LI , CHEN Ji-Zheng, ZHANG Fa-Ke, NIU Zheng-Ji. ZHU Peng-Jiu and LI Ji-XiResearch Laboratory 403-1Xi'an Electronic University of Science and Technology;Effects of Millimeter-Microwave on the Peripheral Blood White Cells and the CFU-C of BMC in Mice Injured by ~60Co γ-Rays[J];Journal of The Fourth Military Medical University;1990-02
3 ZHAO Qing\|liang WANG Jing\|he\ LI Dan\ DONG Shen (Precision Engineering Research Institute,Harbin Institute of Technology,Harbin 150001,China);Innovations and developments of scanning probe microscopy[J];JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY;2000-01
4 CAO Xiao zhe,WANG De wen,ZHAO Mei lan,CUI Xue mei (Institute of Radiation Medicine, Academy of Military Medical Sciences,Beijing 100850,China);Study on the ultrastructural changes of the adrenal gland in rats irradiated by electromagnetic pulse(EMP)[J];Journal of Chinese Electron Microscopy Society;2002-01
5 XIONG Lan 1, SUN Cai xin 1, LIAO Rui jin 1, HU Li na 2, LI Da qiang 2 (1. College of Electrical Engineering,Chongqing University,Chongqing 400044,China; 2. Second Affiliated Hospital of Chongqing Medical University,Chongqing;Elementary Exploration of the Mechanism and Applicable Prospects of Membrane Electroporation[J];JOURNAL OF CHONGQING UNIVERSITY(NATURAL SCIENCE EDITION);2000-04
6 ZHAO Mei lan 1, CAO Xiao zhe 1,WANG De wen 1, LI Chuan lu 2, LIU Cun hua 2,PENG Guang shun 2,TAN Qi mei 2 (1 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China; 2 College of Science, National U;High Field Strength EMP with Its Applied Study in Biology[J];Journal of National University of Defense Technology;2000-S1
7 Cao Xiaozhe, Zhao Meilan, Wang Dewen. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850;INFLUENCE OF EMP IRRADIATION ON LDH, AST, CHE, K+ AND Na+ EN SUPERNATANT OF CULTURED RAT PI- TUITARY CELLS[J];Medical Journal of Chinese People's Liberation Army;2002-10
8 HE Kun, ZHANG De_Tian, ZHANG Xue_Min, YANG Yi, ZHOU Tao, ZHANG Sa (Instrumental Analysis Center, Academy of Military Medical Sciences, Beijing 100850, China);Application of atomic force microscope to biomedicine[J];Bulletin of The Academy of Military Medical Sciences;2002-02
9 WANG Bao_fang1,LU Xiu_xiang1,XU Xiao_bo1,ZHANG Lin2 (1.School of Medicine,Henan University,Henan 475001,China);Effects of 900 MHz Pulse Microwave Electromagnetic Radiation on Learning Capacity and Memory Abilities of Rats[J];Journal of Labour Medicine;2003-04
10 DU HONG-YAN; ZHANG WEI-NING; ZHANG ZI-GUI;SHAO YUE; WANG LIANG-BIN; WANG JIN-XI;AND WU FU-MEI(Department of Biological Science and Technology,Nanjing University,Nan jing 210093)(State Key Laboratiry of Coordination Chemistry,Nanjing 210093);CORRLEATION BETWEEN ABILITY OF LEARNINGMEMORY AND SYNAPTOSOMAL FREE[Ca ̄(2+)]_ i IN MICE OF DIFFERENT AGE[J];ACTA PHYSIOLOGICA SINICA;1996-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved