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《Journal of Plant Protection》 2003-03
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Effect of the metabolites of Pseudomonas sp. 20 # -5 on Phytophthora infestans

Lan Xiqian Zhou Zeyang Hu Junhua Bi Chaowei Yang Xingyong (The Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University, Chongqing 400716, China)  
In order to study the characteristics of the antibiotic-producing Pseudomonas sp. strain 20# -5, inhibition spectrum of the metabolites was investigated by in vitro test. The active components were extracted by methanol and then isolated by chromatogram, and the fermentation conditions were optimized. The results showed that the metabolites can strongly inhibit the growth of B. thuringiensis , B. subtilis , B. cereus , S. aureus and Phytophihora infestans , which causes the destructive potato later blight. Yersinia sp. can be weakly inhibited, but none of the X. campestris , E. coli, S. arizonae, and Klebsiella sp. was inhibited. After isolating the concentrated samples by gel chromatograph and reverse chromatograph more than one active component existed in the metabolites. The optimal fermentation conditions were the followings: 10 ml of LB liquid medium with the initial pH8.0 in 100 mi-flask and shaking it at 28℃ for 4 days.
【Fund】: 渝科委计[2000]34号(重庆市科委院士基金);; 教育司[2000]65号(教育部骨干教师资助计划)
【CateGory Index】: S435.32
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