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Mechanism of action of the novel herbicide ZJ0273

CHEN Jie YUAN Jun Zhejiang Chem-tech Group CO,Ltd,Hangzhou 310023,China LIU Ji-dong WU Jun Zhejiang University,Hangzhou 310010,China FU Qun-mei Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032,China  
The mechanism of the novel herbicide ZJ0273 is studied with the conventional bioassay and bio- chemical methods.The results indicated that the addition of 5 mg/L of 3 branched-amino acids to sorghum seedling cultures incubated in the presence of ZJ0273 completely alleviated herbicide-induced stem growth in- hibition.But each of Val,Ile and Leu single was only partly effective in reversing ZJ0273-induced growth in- hibition at the same concentrations.The IC_(50) values of ZJ0273 and bispyribac-sodium were respectively over 100 mg/L and in the ranges of 10~(-5)-10~(-4)mg/L in vitro,so ZJ0273 showed little inhibition effect on acetolac- rate synthase (ALS).ALS was greatly inhibited by ZJ0273 in vivo,and the activity of ALS was lowered when the treat time increasing.It is concluded that ZJ0273 inhibited biosynthesis of the essential branch amino acids and acted as an ALS inhibitor.However,the mode of action of ZJ0273 differs from the other typically commer- cial ALS inhibitors,such as sulfonylurea and pyrimidine salicylic acid herbicides.Its mechanism is similar to a pre-herbicide,and activated by the metabolic processes in the plants.
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