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《Acta Phytophylacica Sinica》 2009-03
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Sensitivity of Monilinia fructicola isolates to thiophanate-methyl and boscalid

Fan Jinyan Fang Yali Guo Liyun(Department of Plant Pathology,China Agricultural University,Beijing 100193,China)  
In this study,an investigation was carried out to detect the sensitivity of Monilinia fructicola isolates collected from different areas in north China to common fungicide,thiophanate-methyl,and new fungicide,boscalid,with traditional method for assessing fungicide sensitivity of pathogen,as well as the genetic diversity of these isolates were analyzed using 12 ISSR primers.The results showed that among 74 tested Chinese isolates,5 isolates were highly resistant and 2 were lowly resistant to thiophanate-methyl.Alignment results of β-tubulin sequences of Chinese two high-resistant and one low-resistant isolates after sequencing showed that they had same point mutations as those of isolates from California,USA.The frequency distribution of the EC50 values of boscalid to 45 isolates from different areas of China,USA,New Zealand and France was a unimodal curve,ranging from 0.059-0.320 μg/mL,with a mean value of 0.174±0.064 μg/mL;there was no correlation between the UPGMA group based on ISSR analysis of isolates with their locations as well as with their resistance to thiophanate-methyl.
【Fund】: International Foundation of Science(D3938-1);; 自然科技资源共享平台(2005DKA21207-12)
【CateGory Index】: S436.6
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