Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Phytophylacica Sinica》 2009-04
Add to Favorite Get Latest Update

cDNA cloning and quantitative analysis by real-time RT-PCR of tra-1 gene in Ovomermis sinensis

He Junfei Wang Weina Zhou Qingchun Liu Xusheng Wang Guoxiu (Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Sciences,Huazhong Normal University,Wuhan 430079,Hubei Province,China)  
Tra-1 gene was partially cloned from mermithid nematode by using RT-PCR and 3′-RACE for the first time. The 5′ truncated cDNA was 800 bp in length and encoded a putative protein of 195 amino acids,which had high homology with other members of GLI zinc-finger transcription factors in the zinc-finger domain. The identities were 70%-77%. The transcript levels of tra-1 were analyzed by real-time fluorescent quantitative PCR. The results showed that the tra-1 transcript presented high levels in the female adults (P0.01),then dropped observably during the stages of embryos and mated female adults,while were significantly higher than that in other groups (P0.05). However,the expressions among pre-parasites,parasites,post-parasites and male adults,had no remarkable differences (P0.05). Different numbers of nematode hosted in host didn’t infect the tra-1’s transcription obviously (P0.05). Studying on the basis of quantitative results,the author presumed the TRA-1A had a share in the control of the gonad development,the oogenesis and embryonic development of the O.sinensis.
【Fund】: 国家自然科学基金(30870350 30740064)
【CateGory Index】: S476.15
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 REN Shuang WANG Wei-Na ZHAO Na-Na AI Huan WANG Guo-Xiu(College of Life Sciences,Central China Normal University,Wuhan 430079,China);Research progresses and prospects of nematode,Ovomermis sinensis[J];Chinese Journal of Applied Entomology;2011-03
2 GUO Qing,WANG Zhong-yue*,KONG Fan-fang,WU Yan-xia (State Key Laboratory for Biology of Plant Diseases and Insect pests,Institute of Plant Protection, CAAS,Beijing 100193,P.R.China);Application of Real-time Fluorescence Quantitative PCR on Plant Pests[J];Jiangxi Plant Protection;2011-02
3 Ren Shuang Chen Chong Liu Xusheng Wang Guoxiu (Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Huazhong Normal University,Wuhan 430079,Hubei Province,China);Molecular cloning and expression analysis of vasagene inOvomermis sinensis[J];Acta Phytophylacica Sinica;2011-04
【Citations】
Chinese Journal Full-text Database 4 Hits
1 GAO Yuan,WANG Guo-Xiu ** ,CHEN Si-LiCollege of Life Sciences, Central China Normal University, Wuhan4300 79, China;Preliminary analysis on dissoluble protein of Ovomermis sinensis adults by 2-D PAGE[J];Acta Zoologica Sinica;2004-01
2 JIAO Bao-Wei WANG De-Shou DENG Si-Ping(School of Life Science, Southwest Normal University Chongqing 400715, China);The Approach of TSD in Reptile[J];Chinese Journal of Zoology;2002-04
3 LI Jun-li~1,WANG Guo-xiu~(1),WANG Wei~2,LIU Xu-sheng~1,QIAO Zhi-xian~1,ZHAO Shan~1(1.College of Life Science,Central China Normal University,Wuhan 430079;2.College of Plant Science and Technology,Huazhong Agriculture University,Wuhan 430070);Changes of the morphological and chemical substances of the Ovomermis sinensis during gonadial development[J];Journal of Central China Normal University(Natural Sciences);2006-02
4 LIU Hui,CHEN Si-Li,WANG Guo-Xiu(Central China Normal University,Laboratory of Molecular Biology,Wuhan 430079,China);Transformer Genes and Sex Determination of C.elegans and D.melanogaster[J];Hereditas(Beijing);2005-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Mao-Xian,LI Yang,WANG Guo-Xiu **College of Life Sciences, Central China Normal University, Wuhan 430079, China;Changes in the contents of reducing sugar, trehalose and free fatty acids in the haemolymph of Helicoverpa armigera infected by Ovomermis sinensis[J];Acta Zoologica Sinica;2005-02
2 HUANG Min Yi ZHANG Yu Hui WANG Hong Yuan (College of Life Science, Shaanxi Normal University, Xi′an 710062, China);Sex Determination in Non-mammalian Vertebrates[J];Chinese Journal of Zoology;2004-04
3 ZHOU Lin-yan,ZHANG Xiu-yue,WANG De-shou * (Southwest China Normal University,Chongqing 400715,China);Advances on Molecular Mechanism of Sex Determina-tion and Differentiation of Vertebrates[J];Zoological Research;2004-01
4 LIU Jia LI Xin-Yi ZHANG Yu-Hui College of Life Science,Shaanxi Normal University,Xi′an 710062,China;Sex Determination-related Genes in Amphibians[J];Chinese Journal of Zoology;2011-06
5 LI Zhi-Qiang~ 1,2,3 , WANG Mao-Xian~1, YUAN Jun-Lin~1, CHEN Guo-Sheng~ 1, (1. College of Life Sciences, Central China Normal University, Wuhan 430079, China; 2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China);Activity changes of phenoloxidase in haemolymph of Helicoverpa armigera parasitized by Ovomermis sinensis and its purification[J];Acta Entomologica Sinica;2006-05
6 YUE Hua-Mei, WANG Jiang-Yi, WANG Hong-Tao, WANG Guo-Xiu*(College of Life Science, Central China Normal University, Wuhan 430079, China);Research progresses and applications of nematode, Ovomermis sinensis[J];Chinese Bulletin of Entomology;2006-06
7 REN Shuang WANG Wei-Na ZHAO Na-Na AI Huan WANG Guo-Xiu(College of Life Sciences,Central China Normal University,Wuhan 430079,China);Research progresses and prospects of nematode,Ovomermis sinensis[J];Chinese Journal of Applied Entomology;2011-03
8 ZHAO Weimin1,HOU Tiande2,CHENG Fang2(1.Department of Biological Sciences,Qinghai University,Xining 810016,China; 2.College of life Science,Northwest Normal University,Gansu,Lanzhou 730070,China);Breeding habit and embryonic development of Elaphe dione[J];Journal of Qinghai University(Nature Science);2011-01
9 ZHANG Qiang~ 1,2 ,XIE Feng~ 1* ,JIANG Jian-ping~1, ZHENG Zhong-hua~1,XU Jun-xiao~ 1,2 ,CHENG Gui-ying~1 (1.Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041; 2.Graduate School of the Chinese Academy of Sciences);A Review on Cold Adaptation of Cold-blood Vertebrate[J];Sichuan Journal of Zoology;2006-02
10 DING Guo-hua1,YANG Jing1,2,XU Da-de2,3,LI Hong1,JI Xiang1 (1College of Life Sciences,Nanjing Normal University,Nanjing 210046,China; 2Hangzhou Key Laboratory for Animal Adaptation and Evolution,Hangzhou Normal University,Hangzhou 310036,China; 3Department of Biology,Zhaoqing University,Zhaoqing 526062,Guangdong,China);Temperature-dependent sex determination in reptiles[J];Chinese Journal of Ecology;2010-10
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Guo Xiu CHEN Qu Hou (College of Life Sciences, Central China Normal University, Wuhan?430079, China) CHEN Guo (Biology Control Laboratory,Chinese Academy of Agricultural Sciences,Beijing?100081,China);IN VITRO CULTIVATION OF THE ENTOMOGENOUS NEMATODE OVOMERMIS SINENSIS[J];Acta Zoologica Sinica;2001-02
2 L Dao-Yuan 1, SONG Ping [DK3]1** , CHEN Yun-Gui 1, PENG Mao-Yu 1,GUI Jian-Fang 2 1. School of Life Sciences Wuhan University, Wuhan 430072, China 2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;Expression of gene vasa during gonadal natural sex reversal of ricefield eel Monopterus albus[J];Acta Zoologica Sinica;2005-03
3 XU Hong-Yan 1,2, PENG Jin-Xia1, GUI Jian-Fang1, HONG Yun-Han 1,2**1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China2. Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260;Gibel carp germ cell marker Vasa: cDNA cloning and its antibody preparation[J];Acta Zoologica Sinica;2005-04
4 WANG Jiang-Yi,XU Fen,LIU Xu-Sheng,WANG Guo-Xiu**College of Life Sciences,Central China Normal University,Wuhan 430079,China;Molecular phylogeny of entomopathogenic nematodes(Mermi-thidae) inferred from DNA sequences of 18S rDNA,28S rDNA and COI genes[J];Acta Zoologica Sinica;2007-05
5 HU Chong-Jiang,WU Feng-Rui,LIU Zhi-Hao,HUANG Bao-Feng,ZHANG Yao-Guang,WANG De-Shou Key Laboratory of Eco-environments in Three Gorges Reservoir Region(Ministry of Education),Key Laboratory of Aquatic Science of Chongqing,School of Life Science,Southwest University,Chongqing 400715,China;Molecular cloning and expression of two isoforms of Vasa gene in Southern catfish Silurus meridionalis[J];Acta Zoologica Sinica;2008-06
6 CHEN Hong(College of Animal Science and Veterinary Medicine, Northwest Science and Tech nology University of Agriculture and Forestry, Yangling, Shaanxi 712100,China);Modern Biotechnology and Animal Breeding[J];JOURNAL OF YELLOW CATTLE SCIENCE;2000-04
7 CHAI Chun-Li QIAN Ping GUAN Guo-Ping XU Wei LI Wen-Xue XIA Qing-You LU Cheng~* (Key Laboratory of Sericulture of the Agriculture Ministry,College of Sericulture and Biotechnology,Southwest University,Chongqing 400716,China);Studies on Structure and Expression Profile of vasa Gene in Silkworm[J];Science of Sericulture;2006-04
8 Wang Wenzheng, et al. (Burn Unit, First Teaching Hospital);STUDIES ON THE CULTIVATION OF EPIDERMAL CELLS AND TRANSPLANTATlON: I.GROWTH OF CULTURED HUMAN EPIDERMAL KERATINGCYTES IN THREE CULTURE MODELS.[J];Academic Journal of Second Military Medical University;1985-06
9 CHEN Guo JIAN HENG REN HUI-FANG(Biological Control Laboratory, Chinese Academy of Agricultural Sciences, Beijing)PAN CANG-SANG(Parasitology Research Laboratory, Xiamem University, Fujian);A NEW SPECIES OF PARASITIC NEMATODE OF ARMY WORM: OVOMERMIS SINENSIS SP. NOV. (NEMATODA: MERMITHIDAE)[J];Acta Zootaxonomica Sinica;1991-03
10 CHEN Yun-gui1, YE Ding1, SONG Ping~1,* , LV Dau-yuan1, GUI Jian-fang2(1. Key laboratory of the Ministry of Education for Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China;2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, China);Expression and Distributing of vasa Gene During Gametogenesis of Goldfish (Carassius auratus)[J];Zoological Research;2005-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Ming, \ NIE Liu wang, \ GUO Chao wen (Department of Biology, Anhui Normal University, 241000, Wuhu, Anhui, China);The Karyotype and NORs of Ocadia sinensis From China[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);1999-01
2 WANG Guo Xiu CHEN Qu Hou (College of Life Sciences, Central China Normal University, Wuhan?430079, China) CHEN Guo (Biology Control Laboratory,Chinese Academy of Agricultural Sciences,Beijing?100081,China);IN VITRO CULTIVATION OF THE ENTOMOGENOUS NEMATODE OVOMERMIS SINENSIS[J];Acta Zoologica Sinica;2001-02
3 WANG Mao-Xian,LI Yang,WANG Guo-Xiu **College of Life Sciences, Central China Normal University, Wuhan 430079, China;Changes in the contents of reducing sugar, trehalose and free fatty acids in the haemolymph of Helicoverpa armigera infected by Ovomermis sinensis[J];Acta Zoologica Sinica;2005-02
4 CHEN Guo JIAN HENG REN HUI-FANG(Biological Control Laboratory, Chinese Academy of Agricultural Sciences, Beijing)PAN CANG-SANG(Parasitology Research Laboratory, Xiamem University, Fujian);A NEW SPECIES OF PARASITIC NEMATODE OF ARMY WORM: OVOMERMIS SINENSIS SP. NOV. (NEMATODA: MERMITHIDAE)[J];Acta Zootaxonomica Sinica;1991-03
5 CHEN Wu-guo, LUO Qi-gui, WANG Dan, GAO Yuan (College of Life Science, Central China Normal University, Wuhan 430079);Study on hemolymph pathology of Heliothis armigera infected by Ovomermis cinensis[J];Journal of Central China Normal University(Natural Sciences);2002-03
6 Yan Yunjun Bao Xuechun(College of Life Sciences, Central China Normal University, Wuhan430079);STUDIES ON THE LIFE CYCLE OF AGAMERMIS CHANGSHAENSIS[J];JOURNAL OF CENTRAL CHINA NORMAL UNIVERSITY(NATURAL SCIENCES);1997-03
7 XIE Chao, ZHAO Tong Yan, YANG Fa Qing, DONG Yan De, LU Bao Lin (Institute of Epidemiology,Academy of Military Medical Sciences, Beijing 100071,China).;Preparation and staining of serial paraffin slices of mosquitoes.[J];ENTOMOLOGICAL KNOWLEDGE;2000-05
8 GAO Yuan,WANG Guo-Xiu ** ,LIU Zhong-Lai (Laboratory of Zoology,Central China Normal University,Wuiversity,Wuhan 430079,China;;An Economic and Convenient Method for Two-dimensional Electrophoresis[J];Progress In Biochemistry and Biophysics;2003-01
9 Zheng Wenhui ,Tan Bingyan,Tang Lifen and Li Zhangwang(The Sun Yat-Sen University of Medical Scientes, Guangzhou, 510089);Determination of Fatty Acids in Nematode by Gas Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1996-01
10 Wang Peichao (Zoo-ecology Research Laboratory.Dept.of Biology,East China Normal University,Shanghai);PROGRESSES OF STUDIES ON ENVIRONMENTAL SEX DETERMINATION IN REPTILES[J];Acta Ecologica Sinica;1989-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved