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《Journal of Plant Protection》 2018-02
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Sensitivity evaluation of Diaporthe citri populations to mancozeb and screening of alternative fungicides for citrus melanose control

Liu Xin;Wang Mingshuang;Mei Xiufeng;Jiang Liying;Han Guoxing;Li Hongye;Institute of Biotechnology of Zhejiang University;Agricultural Bureau of Quzhou City;Hangzhou Xin'an Plant Protection Company Limited;  
To investigate whether Diaporthe citri populations are still sensitive to mancozeb, and to screen more efficient fungicides for citrus melanose control, the sensitivity of 221 D. citri isolates of four commercial orchard populations and one wild population to mancozeb were tested by using the method of inhibition of conidial germination. The antifungal activities and control effect of the fungicides chlorothalonil and captan were assessed under in vitro, greenhouse, and field conditions. The results showed that the average EC50 values of Nanfeng or Quzhou population were 1.11 and 1.01 μg/mL,respectively, which was significantly higher than that of the wild population with an EC50 of 0.90 μg/mL.However, the resistance factor of the least sensitive isolate in this research was 1.8, less than 5. Chlorothalonil or captan had a better effect than mancozeb in inhibiting the conidial germination of D. citri.When applied as protectants before the inoculation, chlorothalonil, captan and mancozeb showed similar control effects against melanose. Though difenoconazole showed lower inhibitory effect against the conidial germination of D. citri, it had a higher inhibitory effect on mycelial growth than mancozeb,chlorothalonil and captan. When mancozeb and difenoconazole were mixed at the mass ratio of 4∶1,their synergistic effect on mycelial growth was exhibited in vitro. Mineral oil could increase the activity of mancozeb and synergistic effect against conidial germination was exhibited when mancozeb and mineral oil were mixed with the mass ratio of 1∶1 250 or 1∶6 250. Taken together, the populations of D.citri in China are still sensitive to mancozeb and mancozeb remains to be an efficient fungicide for melanose control. Adding a moderate amount of difenoconazole or mineral oil into mancozeb may further promote its control effect against citrus melanose.
【Fund】: 国家公益性行业(农业)科研专项(201303023);; 国家现代农业(柑橘)产业技术体系专项(CARS-27)
【CateGory Index】: S436.66
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