Effects of host plants on the activities of some detoxification enzymes and protective enzymes in the meadow moth
Yin Jiao,Feng Honglin,Li Kebin,Cao Yazhong (State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100193,China)
[Objective]Effects of host plants on the activities of some detoxification enzymes and protective enzymes in the meadow moth were explored to provide the basis for studying the physiological mechanisms of the meadow moth,Loxostege sticticalis,to different plants.[Method]In our experiments,the temporal variation of the activities of 3 detoxification enzymes(carboxylesterase,acetylcholinesterase and glutathione-s-transferase) and 3 protective enzymes(superoxide dismutase,peroxidase and catalase) in the midgut of the moths fed with different plants,including lambsquarters,soybean,corn and potato,was tested.[Result]The results indicated that the enzyme activity was significantly affected by different host plants.The detoxification enzyme activity in the moths fed with suitable hosts was higher than those fed with unsuitable ones.However,with the feeding time prolonged,the fast-growing enzyme activity in the moths feeding on non-suitable host plants reduced the difference among the moths feeding on different host plants.And the variation trend of protective enzymes was similar to that of the detoxification enzymes.[Conclusion]The results indicated that the variation of the detoxification enzymes and protective enzymes to different plants is the reason why the larvae are adapted to non-suitable host plants.