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《Plant Protection》 2013-06
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Construction of marker gene-free RNAi vector and genetic transformation of rice mediated resistance to Ricestripevirus

Lei Yang;Li Yu;Wang Biao;Li Hongwei;Li Li;Wang Xifeng;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;  
In this study,a marker gene-free RNA interference(RNAi)vector pCMBIA1301+/-R was constructed with a partial coat protein(CP)gene of RSV.The vectors pCMBIA1301+/-R and pCMBIA1301 were transformed into Agrobacterium tumefaciens"shooter"strain EHA105 by electroporation,respectively.We also attempted to improve the efficiency of genetic transformation in the test.The effects of both rice varieties and density ratio of two recombinant agrobacterium strains on the co-transformation efficiency were investigated.The results showed that the rice variety‘Aichiasahi'(9.07%)was more adaptive to co-transformation system by RNAi vector than that of the variety‘Wuyujing3'(6.59%)and‘Huaidao5'(0%).Additionally,when the density of recombinant agrobacterium E13R and E1301 was3∶1,the co-transformation efficiency was 16.33%,higher than those of the other density ratios,including 2∶1(13.56%),1∶1(9.09%)and1∶2(5.97%).Sixteen marker gene-free plants were successfully obtained in this study.
【Fund】: 转基因生物新品种培育重大专项(2012ZX08009001 2009ZX08001-013B)
【CateGory Index】: S435.111.4
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