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《Plant Protection》 2015-01
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Establishment and application a method for testing the sensitivity of Peronospora parasiticato metalaxyl

Yang Lijuan;Zhao Honghai;Liang Chen;Guo Yanrui;Shi Sujuan;Liu Wei;Key Lab of Integrated Crop Pest Management of Shandong Province,College of Agronomy and Plant Protection,Qingdao Agricultural University;  
A new method,cotyledonin vitroimmersion method,was established and used for detecting the sensitivity of Chinese cabbage downy mildew pathogen(Peronospora parasitica)to metalaxyl.After soaked for 3hin metalaxyl with 0.005% Tween 20 plus 0.1% acetone,the cotyledons in vitro were artificially inoculated with sporangium suspension(1×104 sporangium/mL),then incubated in the moist chamber,and the disease severity was checked after 6dof culture.Sensitivity of 50 P.parasiticastrains to metalaxyl was tested.The results revealed that sensitivity of some strains decreased significantly and resistant subpopulations emerged.The studies indicated that the method was suitable for the sensitivity detection of P.parasiticato fungicides and in accord with practice for its good repeatability and higher sensitivity and precision.
【Fund】: 公益性行业(农业)科研专项(201003004);; “泰山学者”建设工程专项经费
【CateGory Index】: S436.341.12
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