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《Plant Protection》 2015-05
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Molecular cloning and sequence analysis of superoxide dismutase gene from Tomicus yunnanensis

Zhao Yanming;Zhu Jiaying;Ze Sangzi;Zhao Ning;Yang Bin;Key Laboratory of Forest Disaster Warning and Control in Yunnan Province,Southwest Forestry University;Yunnan Vocational College of Forestry;  
Superoxide dismutase(SOD)is a kind of antioxidant enzymes,existing widely in biological groups.It plays a key role in insects' antioxidant protection system.A cDNA encoding Cu/Zn-SOD was first isolated from Tomicus yunnanensis using rapid amplification of cDNA ends(RACE)strategy.This gene was 768 bp in fulllength with an open reading frame(ORF)of 411 bp,which encoded153 amino acid residues.Its predicted molecular weight and isoelectric point were 15.8 ku and 5.68,respectively.The deduced amino acid sequence of T.yunnanensis SOD showed the highest similarity(93%)with that of Dendroctonus ponderosae,and had 67%-71%similarity with other insects,including Tribolium castaneum,Riptortus pedestris,Triatoma infestans,Nasonia vitripennis,Camponotus floridanus,Papilio xuthus,Helicoverpa armigera,Drosophila virilis and Musca domestica.Two typical structure and functional domains of Cu/Zn-SOD(GFHIHEFGDNT42-52 and GNAGGRLACAVI136-147)were located in the deduced amino acid sequence.Several highly conserved motifs including Cu,Zn binding sites H(44),H(46),H(61),H(118)for Cu binding,and H(61),H(69),H(78),D(81)for Zn binding,and Cys55 and Cys144 for the only intrachain disulfide bond were also identified in T.yunnanensis Cu/Zn-SOD.Phylogenetic tree indicated that T.yunnanensis Cu/Zn-SOD belonged to icCu/Zn-SOD family.The real-time quantitative PCR showed that icCu/Zn-SOD gene expressed at different developmental stages and in different tissues of T.yunnanensis,but the expression profiles were different.
【Fund】: 国家自然科学基金项目(31260179);; 国家林业局林业公益性行业科研专项(201004067)
【CateGory Index】: S763.38
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