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ON A PEONY ISOLATE OF TOBACCO RATTLE VIRUS (TRV—Pa)

Wei Ningsheng Wu Yunfeng (Department of Plant Protection, Northwestern Agricultural University, Yangling)  
A virus obtained from yellow mosaic and pollen sterile peony (Paeonia suffruticosa) in the campus of Northwestern Agricultural University has been identified as a Pa isolate of tobacco rattle virus (TRV-Pa) based on host range, stability,particle morphology, serology and components of amino acids.TRV-Pa can infect 21 plant species among 5 families by sap inoculation.Virus TDP is 80℃, DEP is 10~(-3)-10~(-4) and L is over 40 days. Under EM, TRV-Pa presents two different lengths(35-80nm and 125-200 nm).in electrophoresis TRV-Pa gives two bands (band 1 and band 2) with Rf 0.25 and 0.26-0.28 respectively. Their moving speeds are-1.57-1.6×10~(-5) and -1.7——1.75×10~(-5)cm·sec~(-1) v~-·and the marker BYDV's speed is -1.43——1.45×10~(-5)cm~2·sec~(-1)·v~(-1).Pa-RNAs' MW are 2.45×10~6(RNA_(?)) and 0.65×10~6 (RNA_2)by parallel RNAs'MW of brome mosaic virus (BMV) and tobacco mosaic virus (TMV).A simple band of viral protein whose MW is 2.11×10~4 behaves with some protein markers (bovine serum albumin, pepsinun, trypsin and cytochrome C) on 12% PAGE. Viral protein consists of 4 major amino acids (Asp.,Glu.,Thr.and Ser.),and is shortage of Cys..Cytopathological and serological studies of infected peony tissue indicate that TRV-Pa particles exist in abnomal pollen, about 80% pollens are empty and the remaining alive ones are mostly anomalous.For control of TRV transmission through any asexial reproduction in peony, the virus-free materials must be employed.
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