PRODUCTION OF TRANSGENIC CHINESE CABBAGE BY TRANSFORMATIONWITH THE CP GENE OF TURNIP MOSAIC VIRUS
ZHU Chang xiang, SONG Yun zhi, ZHANG Song, GUO Xing qi , WEN Fu jiang (College of Life Sciences, Shandong Agricultural University, Taian 271018,China)
Using cotyledonary petioles of Chinese cabbage [ Brassica campestris L. ssp. pekinensis (Lour) Olsson] "Fushan Dabaotou" as material, we investigated the factors affec ting plant regeneration and genetic transformation mediated by Agrobacterium tumefaciens . As a result, an efficient system for plant regeneration and genetic transformation was established, and the TuMV CP gene was introduced into Chinese cabbage. Preliminary molecular analysis indicated that five of the 32 putative transgenic plants were PCR positive. The five plants were then subsequently analyzed by Southern blot. The results showed that the TuMV CP gene was integrated into the chromosomes of the plants. Northern blot analysis and ELISA test indicated that the TuMV CP gene was expressed at both transcriptional and translational levels. Further studies indicated that the TuMV CP gene was segregated at a 3∶1 ratio in T 1 generation. Resistance assay revealed that the plants of T 1 generation showed high level of resistance to TuMV infection.