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《Acta Phytopathologica Sinica》 2003-02
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Study on the blast resistance of transgenic rice plants with two antifungal protein genes

YANG Qi-yun;XU Xin-ping;ZHU Xiao-yuan;FENG Dao-rong;LI Bao-jian Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China Biotechnology Research Center, Zhongshan University, Guangzhou 510275, China  
Twenty two transgenic rice lines with basic chitinase gene and β-1, 3-glucanase gen were screened for blast re- sistance by. conducting seedling screening, resistant spectrum test, and field resistance test in the blast are nursery. A series of transgenic rice plants with improved resistance were obtained. Among them, seven transgenic lines with high resis- tance were selected from transgenic rice line F4-9, and these highly resistant lines also expressed stable resistance in the R7 generations based on the tests of resistant spectrum to blast fungus in greenhouse and the neck resistance in blast nursery. The transgenic rice lines with high resistance and quality were obtained successfully in this experiment. The data showed that genetic engineering was a powerful approach to improve the resistance of rice variety to blast and alleviate the negative correlation between blast resistance and quality in southern rice area.
【Fund】: 国家“863”高新技术发展计划资助项目(10101-02-02);; 广东省自然科学基金(970674)
【CateGory Index】: S435.111.2
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