Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Phytopathologica Sinica》 2005-02
Add to Favorite Get Latest Update

Preparation and application of non-radioactive, biotin-labelled PSTVd-cDNA

LU Dian-qiu 1, LI Xue-zhan 1, YU De-cai 1, HU Lin-shuang 1 , YANG Xi-cai 2 ( 1Supervision and Testing Center for Virus-Free Potato Seeds of Ministry of Agriculture, Harbin 150086, China; 2Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China)  
pGEM-PSTVd with monomeric PSTVd cDNA (359 bp) was used as a template and the dUTP was partically replaced by biotin-11-dUTP. The cDNA probe was prepared by PCR amplification technique. Colori-metric and chemiluminescent detection for biotinylated probe were used in this test. They were capable of detecting 50 pg purified total RNA by colorimetric assay and 5 pg by chemiluminescent assay. Nonradioactive probes produced with PCR amplification are particularly suitable for practical diagnosis, as they are sensitive and can be rapidly prepared in large quantities. This two assay methods were 26 times and 260 times as sensitivity as R-PAGE respectively. The diagnosis was sensitive, specific, quick and easy to follow.
【Fund】: 国家科技部基础研究重大项目(2001CCC02900)
【CateGory Index】: S435.32
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved