Morphological changes of the witches' broom seedlings of Paulownia tomentosa treated with methyl methanesulphonate and SSR analysis
CAO Xi-bing,FAN Guo-qiang,ZHAI Xiao-qiao(Institute of Paulownia,Henan Agricultural University,Zhengzhou 450002,China)
Witches' broom is one of the most serious diseases in Paulownia production.Morphological changes of the witches' broom seedlings of Paulownia tomentosa treated with methyl methanesulphonate and SSR analysis were investigated in order to further study the relation between the disease and DNA changes.The results indicated that the diseased seedlings treated with optimal concentration of MMS might turn into the ones without symptom morphologically,but there still existed the phytoplasma in the seedlings treated with 20 mg·L-1 MMS.Not only might MMS of which the concentrations were more than 80 mg·L-1 eliminates the phytoplasma from the seedlings,but also inhibits the seedling growth.DNA base sequences of the healthy,di-seased and the diseased-treated with 20 and 100 mg·L-1 MMS seedlings were the same at SSR level.
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