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《ACTA PHYTOTAXONOMICA SINICA》 1999-04
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Application of ITS sequences of nuclear rDNA in phylogenetic and evolutionary studies of angiosperms

1WANG Jian Bo 2ZHANG Wen Ju 2CHEN Jia Kuan 1(College of Life Sciences,Wuhan University,Wuhan 430072) 2(Institute of Biodiversity,Fudan University,Shanghai 200433)  
Nuclear rRNA genes (rDNA) in angiosperms are arranged in long tandem repeating units, much like those of other higher eukaryotes. Owing to rapid concerted evolution, the repeat units have homogenized or nearly so in most species. The internal transcribed spacer (ITS) of nuclear rDNA is composed of ITS1 and ITS2, which are seperated by 5.8S rDNA. The two spacers, ITS1 (187~298 bp) and ITS2 (187~252 bp), can be readily amplified by PCR and sequenced using universal primers. The sequences contain many variable sites and potential informative sites among related species, and have been proven to be a useful molecular marker in phylogenetic and evolutionary studies of many angiosperm taxa. It can be used not only in classification and phylogenetic inferences at the levels of family, subfamily, tribe, genus and section,but also in reconstruction of reticulate evolution and detection of the speciation via hybridization and polyploidization. But this region may not be useful for resolving phylogenetic relationships among families or taxa of higher hierarchy owing to the rapid variation of the ITS sequences.
【Fund】: 国家自然科学基金
【CateGory Index】: Q949.7
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【References】
Chinese Journal Full-text Database 10 Hits
1 Gao Penghua;Yu Wenjian;Zhao Rui;Wang Jin;College of Forestry, Southwest Forestry University;;ITS Sequence Analysis of 38 Climbing Rose Varieties[J];分子植物育种;2018-04
2 HAN Zhixiao;ZHANG Jun;ZUO Lihui;DONG Yan;WANG Jinmao;Stated-owned Assets Management Bureau,Hebei Agricultural University;College of Forestry,Hebei Agricultural University;;Populus Polymorphism and Cluster Analysis based on SSR and ITS Markers[J];中国农业科技导报;2017-07
3 LI Xiaochen;MA Songmei;College of Science,Shihezi University;;Genetic Variation Structure of the Endangered Plant Amygdalus pedunculata[J];西北植物学报;2017-07
4 Xu Kecheng;Dong Yueli;Yang Zixiang;Wei Yonggui;Huang Qiong;Sun Yuexian;College of Plant Protection,Yunnan Agricultural University;Tropical Eco-agriculture Institute of Yunnan Academy of Agricultural Sciences;Yunnan Wenshan Academy of Agricultural Sciences;;DNA extraction from Cylas formicarius(Coleoptera:Brentidae) by Chelex-100 method and molecular identification of Cylas formicarius in Yunnan[J];植物保护;2017-03
5 MA Yanzhi;Department of Life Sciences,Tangshan Normal University;;Diversity of Schizonepeta tenuifolia Briq.based on AFLP and ITS sequence analysis[J];西北农林科技大学学报(自然科学版);2017-06
6 JIANG Ming;GUO Zhi-ping;ZHANG Yan-ru;XU Xin;ZHU Xin;JIANG Xiao-ying;LV Yi;Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation in Taizhou University;College of Life Science, Taizhou University;College of Medicine and Health, Lishui University;;Cloning and analysis of rDNA ITS sequences from seven kinds of medicinal plants in Goodyera L.[J];中草药;2016-23
7 GAO Zhijuan;ZHUANG Li;College of Life Science,Shihezi University;;Phylogeny and genetic relationship of 9 Haloxylon ammodendron populations in Junggar basin and Alxa Left Banner[J];干旱区资源与环境;2016-11
8 ZHOU Xueli;YANG Lucun;LI Guiquan;TONG Shixian;Tiebujia Grassland Improvement Experiment Station;Northwest Institute of Plateau Biology,Chinese Academy of Sciences;Qinghai Jiushi Chongcao Biotech.Co.Ltd;;Sequence determination and analysis of the ITS region of nuclear ribosomal DNA in Agropyron cristatum(L.) Gaertn[J];干旱区资源与环境;2016-09
9 LI Shaoji;JIANG Xianghui;College of Chemistry and Materials Engineering,Kaili University;;Comparison of Agronomy Properties and DNA Barcoding Identification in Four Different Species of Lonicera[J];北方园艺;2016-05
10 HAN Zhi-xiao;ZHANG Jun;HAN Meng-di;Li Yang;REN Ya-chao;Agricultural University of Hebei;Hebei Entry-exit Inspection and Quarantine Bureau;Hebei Disabled Persons,Federation;;Analysed the Genetic Relationship of Populus and Salix by ITS[J];河北林业科技;2016-01
China Proceedings of conference Full-text Database 3 Hits
1 CHEN Zhi-tong~(1,2),HUANG Qin- lou~2,PAN Wei-bin~(2,3) HUANG Yi- bin~(1,2) (1.College of Life Sciences,Fujian agriculture and forestry university,Fuzhou,350002,China; 2.Agricultural Ecology Research Institute,Fujian Academy of Agricultural Sciences/Fujian Engineering and Technology Research Center for Hilly Prataculture,Fuzhou,350013,China; 3.Minxi Vocational and Technical College,Resource Engineering Department,Longyan 364021,China);Sequence Analysis of nrDNA ITS Region on Pennisetum species(Poaceae)[A];[C];2009
2 CHAO Zhi 1 XIE Hui 2 HUO Ke-ke 3 PAN Sheng-li2 (1 School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515; 2 School of Pharmacy, Fudan University, Shanghai 200032; 3 School of Life Sciences, Fudan University, Shanghai 200433);Identification of Crude drugs from Medicinal Plants of Genus Bupleurum in China Based on Ribosomal DNA ITS Sequences[A];[C];2006
3 Guo Qiongxia, Huang Kehui, Yu Yun (Fujian Entry-Exit Inspection and Quarantine Bureau;Fujian Agriculture and Forestry University);Establishment of PCR-RFLP method based on 18S-26S region of ribosoibosomal DNA for differentiation of Sorghum halepense and S.silk.[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 9 Hits
1 He Tingting;Chai Junhong;Mudanjiang Normal University;;Sequence Analysis of Cymbidium Based on ITS Gene[J];分子植物育种;2017-01
2 Yang Xu;Liu Fei;Zhang Yu;Cheng Yufu;Xue Linbao;Chen Xuehao;College of Horticulture & Plant Protection, Yangzhou University;;Study on the Genetic Diversity of Eggplant Germplasm with SSR Markers[J];基因组学与应用生物学;2016-12
3 ZHOU Xueli;YANG Lucun;LI Guiquan;TONG Shixian;Tiebujia Grassland Improvement Experiment Station;Northwest Institute of Plateau Biology,Chinese Academy of Sciences;Qinghai Jiushi Chongcao Biotech.Co.Ltd;;Sequence determination and analysis of the ITS region of nuclear ribosomal DNA in Agropyron cristatum(L.) Gaertn[J];干旱区资源与环境;2016-09
4 QIAN Xueting;CHEN Wenqiang;DENG Baiwan;XIE Xiuchao;PENG Hao;School of Biological Science and Engineering,Shaanxi University of Technology;Shaanxi Engineering Research Center of Edible and Medicated Fungi;;Analysis of the Genetic Diversity of Auricularia auricular Strains From Qinba Mountains Based on ITS Sequence[J];北方园艺;2016-12
5 LI Sheng-xing;CHEN Jia-lin;ZHANG Tai-kui;HE Xiao;LIU Xiao-zhen;ZHANG Han-yao;Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province,Southwest Forestry University;Key Laboratory of Biodiversity Conservation in Southwest China,State Forest Administration,Southwest Forestry University;;Construction of Phylogenetic Tree Based on ITS Sequence Analysis of some Ficus Species[J];福建林业科技;2016-01
6 ZHANG Xiao-qiu;Sechenbateer;College of Life Science and Technology,Inner Mongolia Normal University;;Study on Pollination Adaptability of Prunus mongolica Maxim.and Prunus pedunculata Pall. to Planting Environment[J];内蒙古林业科技;2016-01
7 LONG Xiangyu;HE Bin;WANG Chuang;HU Yanshi;QIN Yunxia;FANG Yongjun;TANG Chaorong;Rubber Research Institute,CATAS;College of Agronomy, Hainan University;;Genetic Diversity and Phylogenetic Evolution Among 1981'IRRDB Wild Germplasm and Cultivars Using ITS Markers in Rubber Tree[J];热带作物学报;2015-12
8 ZHANG Heqian;YANG Zituo;LI Guifeng;XIAO Shibin;SUN Jijia;YANG Huirong;ZHAO Huihong;XIE Tanghui;HAN Xingpeng;LIU Li;College of Animal Science of South China Agricultural University;School of Life Sciences,Sun Yat-sen University;College of Animal Science,South China Agricultural University∥Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding;College of Animal Science,South China Agricultural University ∥Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding;;Genetic Diversity of Mitochondrial Cyt b Sequences in Pelteobagrus fulvidraco from the Pearl River[J];中山大学学报(自然科学版);2015-05
9 YUN Tao;LI Jia-Man;ZHOU An-Pei;YAN Lu-Xi;ZONG Dan;LI Dan;HE Cheng-Zhong;Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University;Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University;Yunnan Academy of Biodive rsity, Southwest Forestry University;Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University;;Analysis of Phylogenetic Relationship of Populus Based on Sequence Data of Chloroplast Regions[J];植物生理学报;2015-08
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 WANG Xiangdong;MA Yanzhi;KE Shaoying;Tangshan Academy of Agricultural Science;Department of Life Sciences,Tangshan Normal University;;Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR[J];浙江农业学报;2018-04
2 Gao Penghua;Yu Wenjian;Zhao Rui;Wang Jin;College of Forestry, Southwest Forestry University;;ITS Sequence Analysis of 38 Climbing Rose Varieties[J];分子植物育种;2018-04
3 LONG Ting;WANG Jian-ming;LI Jing-wen;FENG Yi-min;WU Bo;LU Qi;College of Forestry,Beijing Forestry University;Institution of Desertification Studies,Chinese Academy of Forestry;;Plant diversity and its environmental explaination in gobi district of northern Qinghai-Tibet Plateau,northwestern China[J];北京林业大学学报;2017-12
4 Li Tao;Wang Hong;Ren Qinyong;Fan Qiaoli;Guo Shihua;Agricultural College, Inner Mongolia Agricultural University;;Sequence Variation of Waxy Gene of Waxy and No-waxy Foxtail Millet and the Similarity on Cereal Crops[J];分子植物育种;2017-12
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