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《Chinese Journal of Health Laboratory Technology》 2008-09
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Analysis of monitoring pathogenic bacterium of bacillary dysentery between 2006 and 2007 in Wuhan

Xiong Yan,Luo Tong-yong,Yu Bin,Cheng Zhi,Jiang Yuan-shan,Long Yi-bing,Zhou Jun-bo(Wuhan Center for Disease Control and Prevention,Wuhan 430015,China)  
Objective:Understanding the biochemistry serology typing,distribution of related virulence genes and drug sensitive state of Shigella,which is the pathogenic bacterium of bacillary dysentery to provide evidence for clinical treat and Control and Prevention in bacillary dysentery.Methods:According to monitoring of pathogenic bacterium of bacillary dysentery in China,we do the separation and identification of pathogenic and bacterium drug sensitive test.And polymerase chain reaction(PCR) were also used to detect related virulence genes of Shigella,in which eight primers such as set1A,set1B,shet2A,shet2B,ial,ipaH,stx1 and virA were used.Results:In 539 specimens of bacillary dysentery by clinical initial diagnosis,17were identified by biochemistry and serology.The rate was 3.2%.15 were of S.sonnei,2 of S.flexneri.ipaH of positive in 27 by PCR,and the rate is 5.0%.the 17 positive straits by separation identification were all ipaH positive.There was some difference in the distribution of related virulence genes between S.flexneri and S.sonnei.All the 17straits were sensitive to amoxicillin and ciprofloxacin.while allwere drug resistance to rifampicin.Five of which were drug resistant to three or more than three kinds of antibiotics.Conclution:Most of the pathogenic bacterium in bacillary dysentery is S.sonnei,secondary is S.flexneri in wuhan between 2006 to 2007.The positive rate is is higher by ipaH-PCR than traditional separation and identification.More importantly,many are drug resistant to the commonly used antibiotics at present.
【CateGory Index】: R516.4
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