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Investigation and evaluation of reference value of reticulocyte in Beijing

HAN Ran,SHOU Hao-chang,WANG Zong-hua(Department of Laboratory Tests,Dongfang Hospital,Beijing University of Traditional Chinese Medicine,Beijing 100078,China)  
Objective:To establish the reference range of 6 reticulocyte parameters of Beijing.Methods:With Sysmex XT-2000i,we randomly studied 207 cases of healthy adults(108 cases of male,female 99 cases) on reference ranges of reticulocyte parameters.At the same time,the classification produced by the XT-2000i and manual methods were compared.Results:RET%:male is 1.31±0.34,female is 1.19±0.32,there was no significant between each age groups(P0.05),unite RET% 1.25±0.36(0.53-1.97);RET#(×1012/L):male is 0.67±0.18(0.33-1.01),female is 0.54±0.16(0.22-0.86)(P0.05);IRF;male is 5.17±2.31(1.41-10.65),female is 4.06±1.59(0.88-7.24),there was significant difference between each age groups(P0.05);on LRF,MRF and HRF,there was significant difference between each age groups on LRF and MRF(P0.05),except HRF(P0.05).And compared with the manual methods:(P0.05).Conclusion:There is no significant difference between reference ranges of 6 reticulocyct parameters with some references.But area,race,sex,analyzer and so on are correlated with the counting results of reticulocyte parameters,so the establishment of this reference range is necessary by laboratory itself for the operation of laboratory and clinic study.At the same time,Compared XT-2000i with the manual methods,the relativity is better.It can replace the manual methods.
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