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《Chinese Journal of Health Laboratory Technology》 2010-05
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Tetracyclines and their metabolites in pork by HPLC

LUO Wan-xia1,ZHAO Qing-rong1,ZHU Yun1,CAI Zeng-xuan2,YANG Qin-de1(1.Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention,Urumqi 830001,China;2.Zhejiang Center for Disease Control and Prevention,Hangzhou 310000,China)  
Objective:A high performance liquid chromatographic method was developed for the determination of tetracyclines and their metabolites in pork.Methods:The samples were extracted with EDTA-Mcllvaine buffer group(pH4.0) and were purified by HLB solid phase extraction column.TCs were successfully separated on a Waters Atlantis T3(4.6×250 mm,5 μm) column using Methyl alcohol-Methyl cyanide-0.02% Formic acid solution as mobile phase at a flow rate of 1.0 ml/min and detected at 355 nm with 35 min.Results:The detection limit of the method was 4.5 μg/kg~22 μg/kg and the quantitative lower limit was 25 μg/kg~80 μg/kg.The linear range is from 25 μg/kg to 1250 μg/kg.Overall recoveries were between 82.5% and 101.9%.RSD value was 0.8%~8.3%.Conclusion:The methods simple,rapid and accurate.It can be used for the determination of tetracyclines and their metabolites in pork.
【CateGory Index】: TS251.7
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 Wang Lei, Xu Zhixiu, Zhang Xiaosong, Shao Xueguang * (Department of Chemistry, University of Science and Technology of China, Hefei 230026);Rapid Determination of 7 Tetracyclines by Reversed Phase High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2003-01
2 YANG Hong-mei,WANG Hao,LIU Yan-qin,YIN Xiao-yan,YAN Long-bao(Haidian District Institute of Products Quality Supervision and Inspection,Beijing 100083,China);Determination of minocycline,Oxytetrocycline,tetracycline,chlortetracycline,methacycline and doxycycline in milk products with LC-MS[J];China Dairy Industry;2007-11
3 WANG Lei, ZHANG Xiao song, XU Zhi xiu, SHAO Xue guang (Department of Chemistry, University of Science and Technology of China, Hefei 230026, China);Determination of Tetracyclines Antibiotics by Reversed-Phase High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2002-01
4 ZHANG Jin-qiang~(1,2,3),LIANG Yan~1,DONG Yuan-hua~2,HUANG Ming-hong~1(1.Croucher Institute of Environment Science,Hongkong Baptist University,Hongkong,China);Toxicity of tetracyclines and their epi-isomers on the luminescent bacterium[J];Journal of Toxicology;2006-05
5 LUO Xiao-yan,LIN Yu-na,LIU Li-zhi.Guangzhou Centre for Disease Control and Prevention,Guangzhou,510080.;STUDY ON THE RAPID DETERMINATION OF 4 KINDS OF ANTIBIOTICS RESIDUES IN AQUACULTURE BY SPE-HPLC.[J];Modern Preventive Medicine;2005-03
6 JI Cai-ni,YUE Zhen-feng,XIE Li-qi,ZHENG Wei-ping,CHEN Pei-jin (Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518067,China);Study on simultaneous detection of 7 tetracyclines residues in aquatic products by high performance liquid chromatography[J];Chinese Journal of Veterinary Science and Technology;2005-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FENG Yu1,SHENG Xuan2,ZHENG Ping2,SUN Hao2,YANG Yan-yan2 (1.Anhui Chlor-Alkali Chemical Group Co.Ltd.,Hefei 230011,China; 2.Anhui Entry-Exit Inspection and Quarantine,Hefei 230061,China);Research Progress on Avermectins Residues Analysis[J];Anhui Chemical Industry;2008-01
2 PAN Xiao-cheng et al (Anhui Agricultural University,Hefei,Anhui 230036);Synthesis and Identification of Ofloxacin Artificial Antigen[J];Journal of Anhui Agricultural Sciences;2006-03
3 WANG Wei-hua et al(Henan Institute of Science and Technology,Xinxiang,Henan 453003);Application of ELISA in the Residue Determination of Sulfonamide[J];Journal of Anhui Agricultural Sciences;2006-06
4 SUN Ming-na et al (Key Laboratory of Crop Quality Amelioration in Anhui Province,Hefei,Anhui 230031);Study on the Development Method of Abamectin Residue Determination[J];Journal of Anhui Agricultural Sciences;2006-12
5 ZHANG Yin-zhi et al (The State Key Lab of Food Science and Technology, Wuxi, Jiangsu 214122);Study on Simultaneous Detection of 3 Tetracyclines Residues in Eggs by High Performance Liquid Chromatography[J];Journal of Anhui Agricultural Sciences;2008-02
6 ZHANG Yin-zhi et al(National Key Lab of Food Science and Technology,Southern Yangtze University,Wuxi,Jiangsu 214036);Study of Rapid Detection on Tetracycline Antibiotics in Food by Using Gold-labeled Reagent Strip[J];Journal of Anhui Agricultural Sciences;2008-27
7 QI Ke-zong~1,ZHU Liang-qiang~2,XU Shi-fu~2,WANG Xue-yan~1,XING Yin-shou~1 (1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036; 2.Anhui Provincial Control Institute of Veterinary Pharmaceuticals and Feed, Hefei 230022);Determination of Recovery Rates of Four Fluoroquinolones Residues in Chicken′s Liver by HPLC[J];Journal of Anhui Agricultural University;2005-01
8 ZHANG Dan-jun1,PAN Xiao-cheng1,CHENG Bao-yan1,ZHAO Rui-hong1,XU Shi-fu2,TANG Chun-lian2,SUN Jun-xia3,HU Xiao-miao1(1.Institute of Animal and Veterinary Science,Anhui Academy of Agricultural Science,Hefei 230031;2.Anhui Provincial Control Institute of Veterinary Pharmaceuticals and Feed,Hefei 230022;3.Tongling Zhenqiang Poultry Company,Tongling 244100);Development of HPLC method for determination of ofloxacin levels in excrements of chicken[J];Journal of Anhui Agricultural University;2009-01
9 MA Shu-Yan ,WU Guo-Juan (Beijing Agricultural College, Beijing 102206;Beijing Institute of Veterinary Drug Control, Beijing 100012);Assay on the Residue of Four Drugs in Animal Products[J];Journal of Beijing Agricultural College;2003-03
10 ZHENG Wei-Hua~1, ZHAO Jian-Zhuang~1,MA De-Ying~2,HOU Shi-Cong~3(~1Beijing Agricultural College, Beijing 102206;~2Xinjiang Agricultural University, Xinjiang,830052;~3China Agricultural University ,Beijing 100094);Development of Determination of Deltamethrin[J];Journal of Beijing Agricultural College;2003-04
China Proceedings of conference Full-text Database 9 Hits
1 FANG Zhong-yi LIU Sui-mei SONG Zhi-chao (Henan provincial supervising agency of veterinary Drugs,Zhengzhou,450008;China);Determination of 4 Tetracyclines in Eggs by High Performance Liquid Chromatography[A];[C];2008
2 SUN Lei ZHANG Li HUANG Yao-ling WANG Xia WANG Shu-huai (China Institute of Veterinary Drug Control,Beijing 100081;China);Determination of Aminoglycosides in Animal Derived Food by UPLC-MS/MS[A];[C];2008
3 SUN Lei~1 ZHANG Li~1 ZHU Yong-lin~2 WANG Shu-huai~1 WANG Xia~1 (1.China Institute of Veterinary Drug Control,Beijing 100081;2.Jiangsu Provincial Test Centre of Animal By-product Quality,Nanjing 210036;China);Determination ofβ-agonists in Animal Derived Food by UPLC-MS/MS[A];[C];2008
4 XIAO Tian-an~1 WU Li-qin~1 FANG Bing-hu~2 DENG Guo-dong~1 (1.Guangdong Institute of Veterinary Drug and Feedstuffs Control,Guangzhou,510230;2.College of Veterinary Medicine,South China Agricultural University,Guangzhou,510642;China);Determination of Maduramicin Residue in Chicken Tissues by HPLC[A];[C];2008
5 YANG Xi-guo CHEN Yu-yan LI Yong-cai ZHANG Jian-xun (Animal Medicine and Feed Supervision Institute of Liaoning Province,Shenyang 110016;China);Determination of Nine Sulfonamides in Feed by HPLC[A];[C];2008
6 LU Guang-ju ZHAOYing SUN Zhi-wen WANGYing (Beijing Institute of Veterinary Drug Control,Beijing 100107;China);Determination of Oxytetracyline,Tetracycline and Chlortetracycline Residues in Egg and Beef by UPLC[A];[C];2008
7 CHEN Rui-qing;LC/MS/MS and Its Application on Veterinary Drug Residue Determination in Food[A];[C];2008
8 ;牛奶中土霉素、四环素、金霉素、强力霉素残留量的测定[A];[C];2010
9 Wei Xinlin~1,Liu Ying~1,Zhang Shaoen~2,Wang Yuanfeng~1 (1.School of Life and Envirnoment,Shanghai Normal University,Shanghai 200234,China; 2.Nankai Biotechnology Limited company,Hangzhou,Zhejiang 310051,China);Synthesis and Identification of Fenvalerate Specific Artificial Antigen[A];Chinese Society of Agricultural Engineering[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Wang Lei, Xu Zhixiu, Zhang Xiaosong, Shao Xueguang * (Department of Chemistry, University of Science and Technology of China, Hefei 230026);Rapid Determination of 7 Tetracyclines by Reversed Phase High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2003-01
2 Yue Zhenfeng~(*1),Qiu Yueming~(2),Lin Xiuyun~(1),Ji Caini~(1) ~1(Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection & Quarantine Bureau of the People′s Republic of China,Shenzhen 518067) ~2(Institute of Inspection Technology and Equipment,Chinese Academy of Inspection and Quarantine,Beijing 100025);Determination of Multi-Residues of Tetracyclines and Their Metabolites in Milk by High Performance Liquid Chromatography-Tandem Positive-ion Electrospray Ionization Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2006-09
3 CAI Ying~1,HAN Yi-yi~1,HE Zhi-jun~2(1.Food Quality Supervision and Inspection Center of China Agriculture Department(Shanghai);2.Shanghai Grain and Oil Products Quality Supervision and Inspection Center Shanghai 200072,China);Determination of Oxytertracycline,Tetracycline,Chlortetracycline Residues in Milk Products by Reversed-Phase High Performance Liquid Chromatography[J];Chemical World;2006-07
4 WANG Lei, ZHANG Xiao song, XU Zhi xiu, SHAO Xue guang (Department of Chemistry, University of Science and Technology of China, Hefei 230026, China);Determination of Tetracyclines Antibiotics by Reversed-Phase High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2002-01
5 JIA Wei, SUN Lu, SHI Xiang guo, CHEN Xiao yan, ZHONG Da fang (Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, China);Measurement of the four tetracycline residues in the milk by high-performance liquid chromatography mass spectrometry[J];Journal of Shenyang Pharmaceutical University;2002-02
6 CHEN Yu _ fang(Guangzhou Exit-Entry Inspection & Quarantine Bureau, Guangzhou 510623, China);Determination of Tetracyclines Residues in Animal Muscle Tissues by SPE-HPLC[J];Journal of Instrumental Analysis;2003-03
7 JI Cai-ni,YUE Zhen-feng,XIE Li-qi,ZHENG Wei-ping,CHEN Pei-jin (Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518067,China);Study on simultaneous detection of 7 tetracyclines residues in aquatic products by high performance liquid chromatography[J];Chinese Journal of Veterinary Science and Technology;2005-10
8 Jiang Dingguo,et al. (National Institute for Nutrition and Food Safety,China CDC,Beijing 100021);Study on the rapid determination of chloramphenicol residues in milk by HPLC[J];Chinese Journal of Food Hygiene;2003-01
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