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《Chinese Journal of Health Laboratory Technology》 2011-11
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The investigation of the CD4~+CD25~+ regulatory T cells in peripheral blood from the patients with pulmonary tuberculosis related diabetes

TAO Ye-hai,DING Yu-yun,CHENG Jiang-nan(The Sixth People′s Hospital of Shaoxing,Shaoxing 312000,China)  
Objective:To determine the percentages of CD4+ CD25+ regulatory T(Treg) cells in peripheral blood from the patients with pulmonary tuberculosis related diabetes,and to investigate the effect on disease development.Methods: A multi-color fluorescence flow cytometry analysis was employed to detect the percentages of Treg cells in peripheral blood from 81 patients with pulmonary tuberculosis related diabetes,63 patients with tuberculosis and 38 healthy donors;and the differences among the three were observed.Results: Treg cells in peripheral blood from the patients with Pulmonary tuberculosis related diabetes group were higher than those in the pulmonary tuberculosis group(t=2.24,P0.05) and healthy control group(t=3.79,P0.01).Treg cells in peripheral blood from the patients with tuberculosis group was higher than healthy control group: t=2.77,P0.01;Treg cells in peripheral blood from the patients with Pulmonary tuberculosis related diabetes in initial treatment stage was higher than in retreatment stage:t=2.19,P0.05;There was a positive relation between blood glucose levels and Treg cells of pulmonary tuberculosis related diabetes patinets(r=0.269,P0.01).Conclusion: The increase of Treg cells in peripheral blood from the patients with pulmonary tuberculosis related diabetes can induce immune tolerance and play a role in the development of the disease.
【CateGory Index】: R521;R587.1
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