Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《》 1983-01
Add to Favorite Get Latest Update


Wang Ai-guo, Luo Guang-hua, Shao Cong-ben, Wu Shu-jun and Guo Jun-yan (Kuo Chun-yen) (South China Institute of Botany, Academia Sinica)  
Superoxide dismutase (SOD) of soybean seeds inhibited the autooxidation of epinphrine and the photochemical reduction of nitrio blue tetrazolium (NBT). The inhibition of the latter reacticn was used as a measure of determining SOD activity the quantitatively. The SOD activity of soybean seeds was found to be inhibited by potassium cyanide, but was not affected by chloroform-ethanol which indicating that the SOD in soyboan seeds was of Cu-Zn type. Soybean SOD was relatively heat-stable, its enzymic activity was lost when kept at 70℃ for 5 min. When the crude extract of soybean SOD was passed through polyacrylamide gel during electrophoresis and stained for enzymic activity with NBT, it was shown to contain five or six distinguishable transparent isoenz yme bands on a blue background. When the crude neutral extract of soybean SOD was purified with chloroformethanol and (NH_4)_2 SO_4 and then chromatographed on Sephadex G-100, the specific activity was shown to increase 13-fold. The purified soybean SOD had the same optical absorption spectra as the bovine blood SOD.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ji-wang,LIU Peng,DONG Shu-ting*,WANG Kong-jun(State Key Laboratory of Crop Biology/Agronomy College of Shandong Agricultural University,Taian 271018,China);Effect of Drought on Root Physiological and Biochemical Characters of Different Maize Cultivars during Seedling Stage WANG Si-si[J];Shandong Agricultural Sciences;2009-06
2 WANG Qi- ming et al (Department of Biology, Shangqiu Teachers College, Shangqiu, Henan 476000 );Effects of Drought- stress on Protective Enzyme Activity and Membrane Lipid Peroxidation in Leaf of Soybean Flowering-poding Period[J];Journal of Anhui Agricultural Sciences;2006-08
3 YUAN Zuo-qing(School of Life Science,Shandong University of Technology,Zibo,Shandong 255049);Effect of Water Stress on Growth of Maize in Germination and Seedling Stages[J];Journal of Anhui Agricultural Sciences;2007-20
4 WANG Lin et al(College of Life Science,Henan Normal University,Xinxiang,Henan 453007);Effect of Hg~(2+) on Isozymes of Peroxidase,Catalase and Superoxide Diamutase in Wheat Seedlings[J];Journal of Anhui Agricultural Sciences;2008-35
5 CHEN Qing-hua(Yongcheng Vocational College,Yongcheng,Henan 476600);Effects of the Drought Stress on the Protective Enzyme Activity and Membrane Lipid Peroxidation of Leaf in Soybean Seedling[J];Journal of Anhui Agricultural Sciences;2009-14
6 CHEN Qing-hua et al(Yongcheng Vocational College,Yongcheng,Henan 476600);Influence of Heavy Metal Stress of Cr6+ on the Physiological and Biochemical Characteristics of Maize Seedlings[J];Journal of Anhui Agricultural Sciences;2009-17
7 CHEN Xiang ming 1, ZHANG Sheng wang 2, MENG Li 2 (1. Hefei Education College, Hefei, Anhui, 230001, China;2. Shandong Agricultural University, Taian, Shandong, 271018,China);PP_(333)DELAYS SENESCENCE OF PEANUT LEAVES[J];Journal of Anhui Normal University(Natural Science);2001-02
8 LI Ji-yuan(Research Institute of Subtropical Forestry,The Chinese Academy of Forestry,Fuyang 311400);Variations of activities for protecting enzymes and superoxide of lipid membrane in Chinese Wingnut tree seedlings(Pterocarya stenoptera) after flooding treatment[J];Journal of Anhui Agricultural University;2006-04
9 LIN Jing (Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014);Effects of Osmotic Stress on Membrane Lipid Peroxidation and Defensive Enzymes Activities of Apricot Leaves[J];Northern Fruits;2001-03
10 ZHU Xiao-long~1;LI Zhen-ji~1;LAI Zhi-hua~1;SONG Ai-qin~1;WANG Jian-lin~2;GUAN Shao-hua~1;YANG Wei-wei~1;ZHENG Ling-feng~2.1 College of Life Sciences,Xiamen University,Fujian Province,361005,P.R.China;2 Administration of Tianbaoyan National Nature Reserve of Fujian,Yong'an City,366000,P.R.China.;Effects of water stress on Tsuga longibracteata seedlings under varied light intensity.[J];Journal of Beijing Forestry University;2007-02
China Proceedings of conference Full-text Database 7 Hits
1 SHEN Yu-xiang~(1,2) GUO Wen-shan ZHOU Ying~1 ZHU Xin-kai~1 FENG Chao-nian~1 PENG Yong-xin~1 (1 Jiangsu Provinical Key Laboratory of Crop Genetics and Physiology/Wheat Research Institute.Yangzhou Unitersity,Yangzhou 225009,Jiangsu,China 2.Department of Chemsitry and Biotechnology.Yancheng Technology College.Yancheng 224002,Jiangsu,China);Effects of Salt Stress on Flag Leaf Senescence after anthesis and Yield in Wheat[A];[C];2006
2 Yao Yuncong, Zhou Xiangdong, Wang Younian, and Liu Yan (Beijing Agricultural college, Beijing 102206);Study on the Identification for Drought-resistance of Apple Germplasm Resources V.Changes of SOD Activity and Relation to Experimental Conditions in Apple Dwarf Cultivars[A];[C];1998
3 Zeng Guoping, Chen Jianxun, Zhang Chongling (Department of horticulture. College of biotechnology, South China agricultural university, Guangzhou, 510642);Effects of Water Stress on Active Oxygen Metabolism in Chinese Kale[A];[C];2000
4 YU Shu-xun, SONG Mei-zhen, FAN Shu-li, YUAN Ri-Hong,HUANG Zheng-Mao(Cotton Research Institute, Chinese Academy of Agricultural Sciences;Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,He′nan 455004, China);The Biochemical Genetic of Short Season Cotton Cultivars(G.hirsutum L.)That Express Early Maturity Without Senescence[A];[C];2005
5 SONG Mei-zhen, YU Shu-xun, FAN Shu-li, YUAN Ri-hong, HUANG Zhen-mao (Cotton Research Institute, CAAS, Key Laboratory of Cotton Genetic Improvement of MOA, Anyang 455004,China);Traits of the Short Season Upland Cotton with the Early Maturity without Senescence[A];[C];2005
6 YAN Mei-Ling, LI Xiang-Dong , WANG Li-Li, ZHOU Lu-ying (College of Agronomy, Shandong Agricultural University, Taian, ,271018, Shandong,China);Effects of different drought stress during seedling stage on some enzyme activities in peanut leaves[A];[C];2006
7 ;Effect of the Stable C1O2 on Storage of Tomato[A];[C];2001
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved