The Effect of Activated Charcoal on the Adsorption of Plant Regulators in Culture Medium
BU Xue Xian;CHEN Wei Lun Institute of Botany, Academia Sinica, Beijing 100044
The effect of activated charcoal (AC) on plant regulators in the medium for plant tissue culture was investigated. The results showed that all of the 12 regulators examined (IAA, IBA, NAA, NOA, 2,4--D, 2,4, 5--T, BA, 2iP, ZT, KT, ABA, and GA_3) in the me- dia could be removed by AC adsorption. The amount of AC needed to remove the normal amount of regulators in the me- dium was very small, i. e., l mg of AC for the removal of more than 100 μg of BA or nearly 100 μg of NAA. Therefore about 0.5～1.0 mg /ml regulators could be removed completely if by adding 0.5～1.0% of AC into the medium. The plant regulator com- binations that could induce axillary shoot proliferation of the shoot tips of Chrysanthemum and shoot formation on leaf fragments of Limmonium, Petunid and Chrysanthemum were no longer effective when AC was added into the medium. Besides, the adsorptive capacity of AC was closely related to the diameter of AC par- ticles.