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《植物生理学报》 1993-04
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Effect of ABA and Environmental Factors on the Degree of Stomatal Opening in Grape Leaf in Ted Tube

CAO Zi--Yi; LI Wei and WANG Xi--Shen Gansu University of Agriculture, Lanzhou 730070  
Treatment with ABA, darkness,low temperature, drought and osmoticpressure decreased leaf stomatal aper-ture of grape plantlets grown in testtube. Owing to a long period of oxpo-sure to high humidity and weak lightthe guard cells of plantlets did not de-velop well, the stomatal pores openedtoo widely, far surpassing the range ofchange in guard cells turgor pressure sothat they failed to adjust and the stom-ata then could not close completely.The experiment was carried out in twocultivars (Ziana and Pioneer). Thewidth of stomatal pores and the rangeof width adjustment of the guard cellsof sand-bed plantlets were 6. 3 and 8.4, 10 and 12. 8μm, respectively.Since the latter was larger than the for-mer, it was large enough to close sothat the stomata could close in thedark. The width of stomatal pores ofthe plantlets grown in test tube was27. 1 and 32. 6 μm which was largerthan the range of guard cells turgorpressure adjustment of 8. 6 and 11. 8μm, so that the too widely openedstomata could not close in the dark.Through appropriate training proce-dures, the stomata can close or open bythe changes in turgor pressure of theguard cells. The percentage of stomata closedin darkness can be taken as an impor-tant physiological index of the adapta-tion of in vitro grown plantlets duringconditioning.
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