Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Photophysiologica Sinica》 2001-05
Add to Favorite Get Latest Update

The Characteristics of Resistance to Photooxidation of Transgenic Rice (Oryza sativa L.) Plants with Maize Genes Coding for C_4 Photosynthesis Enzyme

HUANG Xue Qing JIAO De Mao ( Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences , Nanjing 210014)  
The characteristics of chlorophyll fluorescence and the performance of membrane lipid peroxidation in progenies of transgenic rice plants with maize PEPC, PPDK, NADP ME and PPDK+PEPC genes under photooxidation were studied by comparison with untransformed rice plants ( Oryza sativa L. subsp. japonica Kitaake). After being subjected to photooxidation inducing treatment, Fv/Fm which reflects the primarily photochemical efficiency of PSⅡ, actually photochemical efficiency of PSⅡunder light (PSⅡ) and photochemical quenching (q P) in transgenic rice plants (especially in transgenic rice plants with maize PEPC and PPDK+PEPC genes) decreased less than those in untransformed rice (Fig.2A, B and C), while the non photochemical quenching (q N) increased more (Fig.2D), which suggested that more light energy absorbed by transgenic rice plants was converted into chemical energy and the excessive light energy was dissipated as heat, resulting in the alleviation of photodamage. Meantime, the higher activities of enzymes scavenging active oxygen such as superoxide dismutase (SOD) (Fig.4A), catalase (CAT) (Fig.4B) and peroxidase (POD) (Fig.4C) in transgenic rice plants led to a lesser accumulation of endogenous active oxygen (O -· 2,H 2O 2) (Fig.3A and B). As a result, the malondialdehyde (MDA, a product of membrane lipid peroxidation) content (Fig.3C) was less than that in untransformed rice, and the chlorophyll and protein contents in transgenic rice plants (especially in transgenic rice plants with maize PEPC and PPDK+PEPC genes) decreased less than those of untransformed rice, which showed that there was a stronger tolerance in transgenic rice plants to photooxidation. This work provided the experimental evidence that new varieties with photooxidation tolerant germplasm can be created by introducing the desired germplasm using biotechnology.
【Fund】: 国家重点基础研究发展规划项目 (G19980 10 10 0 )资助
【CateGory Index】: S511.01
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved