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Rapid Determination of Rice Seed Vigor by Ultraweak Chemiluminescence During Early Imbibition

CHEN Wen-Li XING Da HE Yong-Hong (Institute of Laser Life Science, South China Normal University,Guangzhou 510631, China)  
With high-sensitivity single-photon counter, ultraweak chemiluminescence (UCL) study on rice (Oryza sativa L.) seeds stored for different years was carried out. The intensity of UCL was observed to be related to the degree of aging of rice seeds during early imbibition (0-30 min). Rice seeds with longer storage time had lower intensity of UCL during early imbibition. The germination rate of rice seeds showed a significant positive correlation with the intensity of UCL. Singlet oxygen (1O 2) in rice seeds during early imbibition was investigated by chemiluminescence (CL) method using a sensitive CL probe MCLA (2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo pyrazin-3-one). The output of 1O 2 showed a high positive correlation with the germination rate. Analysis based on the experimental results demonstrated that UCL in rice seeds during early imbibition could be attributed mainly to singlet oxygen (1O 2). The UCL technique is a potential way in elaborating a fast, quantitative and non-invasive method for rapid determination of the degree of aging rice seed.
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