Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Photophysiologica Sinica》 2003-01
Add to Favorite Get Latest Update

Ca~(2+)is Involved in the Signal Transduction During Stomatal Movement in Vicia faba L. Induced by Salicylic Acid

LIU Xin 1,2, MENG Fan-Xia 1, ZHANG Shu-Qiu 1*, LOU Cheng-Hou 1 ( 1State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094; 2 Department of Life Science, Laiyang Agricultural College, Laiyang 265200 )  
Using Fluo-3-AM as the Ca 2+ probe, cytosolic free calcium ( i) changes in guard cells of Vicia faba L. in response to exogenous salicylic acid (SA) were measured by confocal laser scanning microscopy (CLSM), and the specificity of i changes in the regulation of stomatal movement by SA were studied. The results showed that i increased within a few seconds after addition of SA, and kept at high levels for several minutes, then declined to the initial level before stomatal aperture changed(Fig.1). We postulated that Ca 2+ involved in the signal transduction of SA regulation of stomatal movement. When Ca 2+ channels in plasma membrane were blocked by extracellular Ca 2+ chelating reagent EGTA (Fig.2), LaCl 3 (Fig.3), or addition of nifedipine(NIF)(Fig.4), the effects of SA on stomatal closure were suppressed differently. Intracellular Ca 2+ chelation with 1,2-bis (2-amino phenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) almost completely abolished the stomatal closure induced by SA (Fig.5). In addition, the extent of i increase by 1 mmol/L SA after pretreatment with NIF was lower than that without NIF pretreatment; almost no i increase was observed after pretreatment with BAPTA(Fig.6). Those results suggest that i increase induced by SA comes mainly from the intracellular storage and partly from the extracellular space.
【Fund】: 国家重点基础研究专项经费 (G19990 1170 0 )资助~~
【CateGory Index】: Q945
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved