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Effect of Epibrassinonde on the Distribution of cAMP and Activity of Plasma Membrane ATPase in Plant Tissue

XU Ru-Juan, HE Yu-Jiong, WU Deng-Ru and ZHAO Yu-Jiu(Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences ,Shanghai 200032)  
The level of cAMP in cucumberhypocotyls was markedly enhanced byepiBR treatment (Fig. 2), and ranparallel to that of hypocotyl elongation, both showing a 6 h lag period(Fig. I). When the membranes ofwheat rootS were incubated with epiBR, the activity of ATPase of themembranes showed no increase, andeven decreased when epiBR concentration was at 2×X10-6 mol/L. But withIAA (5 ×10-5 mol/L) in the reactionsystem, the activity of ATPase waspromoted and was higher than thattreated with IAA alone (Fis. 5). Theradiograph revealed that 125 I-btassinos-terone was distributed mainly in the elongation zone of the epicotyls of mungbeau seedlings, but in the apex andcotyledon bases of cucumber seedlingS.Both were actively growing zones(Fig. 6).
【Fund】: 国家自然科学基金
【CateGory Index】: Q946.5
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