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《Plant Physiology Communications》 2010-05
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Establishment of Tissue Culture and Rapid Propagation Technology of Helianthus tuberosus L.

LU Jie1,2,SONG Yang2,3,WANG Xun2,3,WANG Xiu-Jun2,3,XIAO Hui2,3,WANG Jing1,MU Yan-Jie4,LI Zhu-Gang2,3 1Biochemistry and Molecular Biology of Graduate Academy,College of Life Sciences,Heilongjiang University,Harbin 150080,China;2Biotechnology Institute,Heilongjiang Academy of Agriculture Sciences,Harbin 150086,China;3Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang,Harbin 150086,China;4Microbiology of Graduate Academy,College of Life Sciences,Heilongjiang University,Harbin 150080,China  
Helianthus tuberosus(Jerusalem artichoke) tubers with sprout and shoots with a section of these young stem segments were taken as explants,and were cultured on MS basic medium with several plant growth regulating substances in different concentrations.The technology of tissue culture and rapid propagation on Jerusalem artichoke was researched,and finally filtered out the best combination of technical parameters to establish Jerusalem artichoke regeneration system.The results showed that the optimal explants material for rapid propagation were shoots,positive inoculation(morphology lower down) was the optimal inoculation method.The optimal shoot induction medium was MS+1.0 mg·L-1 6-BA +0.2 mg·L-1 IBA with the induction rate of 95%.The optimal subculture medium was MS+2.0 mg·L-1 6-BA,the optimal strong seedling medium was MS+0.1 mg·L-1 6-BA.The optimal rooting medium was MS+0.2 mg·L-1 NAA,the rooting rate reached 100%,transplant survival rate of 95%,field planting survival rate of 95%.
【Fund】: 黑龙江省科技厅国际合作重点项目(WB07A10)
【CateGory Index】: S632.9;Q943.1
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