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Cloning and Expression Analysis of High-Affinity Potassium Transporter Gene PaHAK1 from Phytolacca acinosa Roxb.

LIN Wan-Huang1,MA Li-Ying1,SU Yi1,HUANG Zhi-Gang1,XIAO Lang-Tao1,ZHOU Qing-Ming2,1Hunan Provincial Key Laboratory of Phytohormones and Growth Development,2College of Agronomy,Hunan Agricultural University,Changsha 410128,China  
To study the mechanism of high affinity potassium absorption in some high-K plants,the PaHAK1 cDNA fragment of 981 bp sequences was cloned from wild Phytolacca acinosa Roxb.by polymerase chain reaction with degenerate primers designed according to conserved HAKs cDNA sequences of Arabidopsis thaliana,Mesembryanthemum crystallinum,Triticum aestivum,Hordeum vulgare and Oryza sativa.The full-length cDNA sequences of PaHAK1 was 2 337 bp which was isolated with rapid amplification of cDNA ends method.The deduced protein was 771 amino acids with molecular weight 86.66 kDa and isoelectric point 7.54.Hydrophobicity plot and topology prediction results indicated the protein had 12–13 putative transmembrane regions.Blastn results showed it was 88% homology to HAK1 of Mesembryanthemum crystallinum.The Real-Time PCR results showed the PaHAK1 was mostly expressed in root of the plant and it could be induced in the low potassium condition.
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