Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Plant Physiology Journal》 2015-04
Add to Favorite Get Latest Update

Expression and Bioinformatics Analysis and Cloning of the Drought-Resistant Gene SiUPF3 in Setaria italica

DAI Yi-Ming;AN Li-Jia;LI Wen-Li;School of Life Science & Biotechnology, Dalian University of Technology;  
Upf3 protein is one of the most important factors in the m RNA surveillance process. This process is a conservative m RNA degradation system which is mainly involved in the degradation of abnormal m RNA in eukaryotic cells. To study drought-tolerant genes, we compared the expression of ‘Jingu34' under drought condition and normal one and filtrated drought-tolerant genes in the level of transcription. In this paper, we got the full length gene which is numbered Si009903 m through searching the sequence within online Setaria italicagenome data. Finally we cloned the coding sequence(CDS) of Si009903 m and named it Si UPF3 for the Upf3 protein it encoded. The CDS of Si009903 m is 1 515 bp and encodes 504 amino acids. Quantitative RT-PCR analysis infered that Si UPF3 is regulated by ABA signal network and significantly upregulated under drought.
【Fund】: 辽宁省科技厅农业攻关项目(2011208001)
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved