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《植物学报(英文版)》 1989-05
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A STUDY OF POLYSACCHARIDES IN ALOE

Wang Shu-xiu Wen Yuan-ying Wang Lei Hu Chang-xu (Institute of Botany,Academia Siaica,Beijing 100044)  
Polysaccharides isolated from fresh leaves of Aloe vera L.var.chinensis(Haw.)Berger, unique species in China,were studied.Purified on columns of Sepharose 6B-CL,three cons- tituents,A_(60),A_(90a)and A_(90b) were got.The M.W.of three polysaccharides were calculated to be approximately 12,000,47,000 and 12,000 respectively by authentic dextrans.A_(60) contained mannose only and A_(90b) contained glucose and mannose in the ratio of 3:4.Smith degrada- tion indicated acetyl butantetraol,2,6-dimethylacetyl mannitol,3,6-dimethylacetyl mannitol and 2,3-dimethylacetyl mannitol for A_(60) and acetyl butantetraol only for A_(90b).Methylation analysis of A_(60) furnished 2,3,6-trimethyl mannose only and methylation analysis of A_(90b) furnished 2,3,6- tri-,2,3,4,6-tetramethylglucose and 2,3,6-trimethylmannose.Both A_(60) an_ A_(90b)were shown β-linkage in IR.From these results structure for A_(60) has been assigned β-(1→4)linked man- nan with partially acetylated in 2,3,or 6 and for A_(90b),β-(1→4)linked glucomannan.Acti- vity against implanted S-180 in mice could not be found in A_(60).But A_(60)was able to promote immunity then could be used for treating the hypoimunity diseases.
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