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《植物学报(英文版)》 1997-02
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ULTRACYTOCHEMICAL LOCALIZATION OF CALCIUM IN THE POLLEN TUBE TRACK OF COTTON GYNOECIUM

Zhang Jin-song and Yang Hong-yuan (Laboratory of Plant Reproductive Biology, College of Life Science, Wuhan University, Wuhan 430072) Zhu Ling and Tong Hua ( Center of Instrumental Analysis, Wuhan University, Wuhan 430072)  
Cotton (Gossypium hirsutum L.) stigma, style and ovule segments were fixed pre-and post-pollination in glutaraldehyde and osmium solutions containing potassium pyroantimonate, processed with conventional dehydration and infiltration procedure, and embedded in Epon 821 resin. To identify the element constitution of the pyroantimonate deposits in the samples, wave-and energy-dispersive X-ray microanalyses were employed, and it was confirmed that the deposits were truly calcium pyroantimonate. Transmission electron microscopic observations revealed that the deposits were more abundant at the whole pollen tube track as compared with the adjacent tissues. They were mainly localized in the apoplast system, i. e. the intercellular matrix of the stigmatic tissue, the outer cell wall layers of stylar transmitting tissue, the intercellular openings and gaps of micropyle, and the degenerated column of nucellus. It was reported that these apoplast spaces were just the positions where pollen tubes grew. The pollen tube tip growing in the micropyle was also rich in calcium, which was especially localized at the new wall, vesicles and mitochondria. All these observations suggested that calcium in the gynoecium tissues may play an suggested important role in the pollen tube growth in situ.
【Fund】: 国家自然科学基金;; 高等学校博士点专项研究基金
【CateGory Index】: S562
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