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Construction of a Trehalose-6-phosphate Synthase Gene Driven by Drought-responsive Promoter and Expression of Drought-resistance in Transgenic Tobacco

ZHAO Hui_Wu, CHEN Yang_Jian, HU Yuan_Lei, GAO Yin, LIN Zhong_Ping [WT6BZ](The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China)  
The trehalose_6_phosphate synthase gene ( TPS ) from Saccharomyces cerevisiae Hansen and the drought_responsive promoter from Arabidopsis thaliana (L.) Heynh. have been cloned by PCR procedure. A plant expression vector with TPS under control of Prd29A has been constructed and used for the genetic transformation of tobacco. The transgenic tobacco with Prd29A/TSP demonstrated TPS expression with increased drought tolerance under drought stress. Some obvious morphological changes including dwarf and fine shoot, lancet_shaped leaves and vigorous auxiliary buds have been observed in a few transformed plants.
【Fund】: 国家自然科学基金资助项目!( 3 9780 0 2 1)&&
【CateGory Index】: Q943.2
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