Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Botanica Sinica》 2001-05
Add to Favorite Get Latest Update

Studies on the Proton Pumping Activity of H~+-ATPase in Tonoplast Vesicles of Populus euphratica

LIU Qun_Lu 1, ZHANG Xu_Jia 2, LI Yi 3, WANG Sha_Sheng 1, JIANG Xiang_Ning 1 * [WT6BZ](1. The Experimental Center of Forest Plant, College of Plant Sciences, Beijing Forestry University,The Ornamental Plant and Tree Breeding and Biotechnology Lab  
Tonoplast_enriched vesicles were prepared from suspension_cultured Populus euphratica Oliv. cells by differential centrifugation and discontinuous sucrose density gradient centrifugation. The properties of the proton pumping activity of H +_ATPases in tonoplast vesicles were studied by acridine orange fluorescent quenching measured at 22 ℃. The proton pumping activity of ATPase was ATP_dependent with apparent Michaelis_Menten Constant ( K m ) for ATP about 0.65 mmol/L. The optimal pH for H +_ATPases activity was 7.5. The proton pumping activity of H +_ATPase could be initiated by some divalent cations, Mg 2+ being highly efficient, much more than Fe 2+ ; and Ca 2+ , Cu 2+ and Zn 2+ were inefficient under the experimental condition. The proton translocation could be stimulated by halide anions, with potencies decreasing in the order Cl - Br -I -F -. The proton pumping activity was greatly inhibited by N_ethylmaleimide (NEM), N,N′_dicyclohexylcarbodiimide (DCCD), NO - 3 and Bafilomycin A 1, but not by orthovanadate and azide. These results demonstrated that the H +_ATPase in the tonoplast of Populus euphratica belonged to vacuolar type ATPase. This work was the first time that tonoplast_enriched vesicles were isolated from Populus euphratica cells.
【Fund】: theNationalNaturalScienceFoundationofChina (39830 32 0 )andthe“9th 5_Year_Plan”Projects (95_0 0 7_0 1_0 5 )
【CateGory Index】: Q945.18
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
2 ShenXin Thomas Teichmenn Wang Yiqin EaiGenben Yu Guangjun Wang Shasheng College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R.China Forestbotanisches Institut, Universitat Gottingen, Biigenweg 2, 37077, Gottingen, Germany Genetics Institute, Chinese Academy of Sciences, Beijing 100010, P. R. China;Gene Response to Salt Stress in Populus euphratica[J];中国林学(英文版);2003-01
3 LIU Ang;WU Wenwei;ZHAO Lei;CHEN Qi;BAI Xiaohua;CHEN Limei;Biotechnology Research Center,College of Life Science and Technology,Chenggong Campus,Kunming University of Science and Technology;Yunnan Environmental Science Institute;;Effects of Magnesium Chloride Pretreatment on the Plasma Membrane H~+-ATPase Activity and Nitrate Absorption Efficiency of Canna generalis in Farmland Wastewater[J];Acta Botanica Boreali-Occidentalia Sinica;2014-01
4 ZHANG Fei,CHANG Jie,JI Qiao-ling(College of Life Sciences and Technology,Xinjiang University,Urumqi Xinjiang 830046,China);Establishment of Suspension Cell Line of Populus euphratica Oliv.[J];Xinjiang Agricultural Sciences;2010-12
5 WANG Jun-Ying, YIN Wei-Lun, XIA Xin-Li(College of Biology Science and Technology,Beijing Forestry University, Beijing 100083, China);Cloning and Structure Analysis of Zinc Finger Protein Gene in Populus Euphratica Oliver[J];Hereditas(Beijing);2005-02
6 LI Xiangyi, LIN Lisha, ZHANG Ximing & ZENG Fanjiang (Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China);Characteristics of Water Status in Populus euphratica Outside the Oases in the Taklamkan Desert[J];Chinese Journal of Applied & Environmental Biology;2007-06
7 ZHOU Hong-hua1,2,CHEN Ya-ning1,2,LI Wei-hong1,2,CHEN Ya-peng1,2(1.Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;2.Key Laboratory of Oasis Ecology and Desert Environment,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China);Characterization of Photosynthesis of Populus euphratica Oliv and Its Microclimate Explanation in Lower Tarim River[J];Journal of Desert Research;2008-04
【Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Huan, ZHU Xiong Wei, WANG Yan Zhi(School of Life Science, Wuhan University, Wuhan 430072, China).;Fluorescence Measurement of Proton Pumping of Tonoplast H + ATPase.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1999-02
2 LIU Qun lu,ZHANG Xu jia 1) ,LI Yi 2) ,WANG Sha sheng,HUANG You guo 1) ,JIANG Xiang ning ** (Experimental Center of Forest Biology,College of Plant Sciences,Beijing Forestry University,Beijing 100083,China; 1) National Labo;Purification of Tonoplast Vescles from Populus euphratica and Its H~+-Pumping Activity[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;2000-03
3 GU Rui-Sheng;JIANG Xiang-Ning;GUO zhong-Chen(Experimental Center of Forest Biology, Beijing Forestry University, Beijing 100083)(Institute of Botany of Botany,The Chinese Academy of Sciences, Beijing 100093);Organogenesis and Plantlet Regeneration in Vitro of Populus euphratica[J];ACTA BOTANICA SINICA;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
2 WU Chun-xia(Key Laboratory of Plant Stress Research,College of Life Science,Shandong Normal University,Jinan,Shandong 250014);Influencing Factors on the Culturing of Plant Suspension Cell[J];Journal of Anhui Agricultural Sciences;2009-01
3 LUO Yi-shai (Xinyang Station of Agricultural Science Institute in Jiangsu Coastal District,Yancheng,Jiangsu 224331);Research Progress of H~+-ATPase Activities in Plant Plasma Membrane and Vacuolar Membrane under Salt Stress[J];Journal of Anhui Agricultural Sciences;2012-03
4 Ding Xia; Chen Xiaoyang; Li Yun; Li Wei.School of Biological Sciences and Biotechnology, Beijing For.Univ., 100083, P.R.China.;Bud regeneration system from leaves of Populus euphratica[J];Journal of Beijing Forestry University;2003-02
5 Zhang Pingdong; Kang Xiangyang; Gao Peng; Han Jin. School of Biological Science and Biotechnology, Beijing For. Univ.,100083,P.R.China.;Comparison of different proliferations of Populus euphratica under in vitro culture.[J];Journal of Beijing Forestry University;2003-06
6 DAI Song-xiang~1; CHEN Shao-liang~1; Eberhard Fritz~2;Andrea Olbrich~2; Christine Kettner~2;Andrea Polle~2;Aloys Hüttermann~2. 1 College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, P. R. China;2 Institut für Forstbotanik, Georg-August-Universitat Gttingen, D-37077, Germany.;Ion compartmentation in leaf cells of Populus euphratica and P.tomentosa under salt stress[J];Journal of Beijing Forestry University;2006-S2
7 Gao Zhun1 Dai Song-xiang1 Chen Shao-liang1,2? Shen Xin1 Wang Rui-gang1 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China 2Key Laboratory of Biological Resources Protection and Utilization in Hubei Province, Hubei Institute for Nationalities, Enshi 445000, P. R. China;Isolation of protoplast from callus of Populus euphratica and H~+ fluxes across plasma membrane under NaCl stress[J];中国林学(英文版);2007-03
8 YAO Na1, ZHANG Zhi-yi1, AN Xin-min1, YANG Kai2 1Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry Uni-versity, Beijing 100083, P. R. China 2Department of Biotechnology, Beijing Agricultural College, Beijing 102206, P. R. China;Establishment of cell suspension line of Populus tomentosa Carr[J];中国林学(英文版);2008-03
9 CHEN Ji-qin~(1), HAN Lie-bao~(1), YANG Chun-qi~(2),LI Xue~(1)(1. Turfgrass Institute,Beijing Forestry University,Beijing 100083,China ;2. Adult Education Department of Ezhou University,Ezhou 436000,China);Effect of different explants on callus induction of perennial ryegrass[J];Grassland and Turf;2005-04
10 Wang Jinmao, Yang Minsheng, Zhen Zhixian, Cao Ying(Agricultural University of Hebei, Baoding 071000, P. R. China); G. Naujoks(Federal Research Centre of Forestry and Forest Products, Waldsieversdorf, Germany);Effect of Exogenous Hormones on Organogenesis and Subculturing of Betula pendula[J];Journal of Northeast Forestry University;2006-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
2 YANG Xiao-yu1,WANG Xiu-feng1,2,HU Chun-chun1,YANG Feng-juan1,2,WEI Min1,2(1.College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China;2.State Key Laboratory of Crop Science,Taian 271018,China);Dynamic Changes of Respiration in Roots and Leaves of Cucumber Seedlings under Nitrate Stress[J];Shandong Agricultural Sciences;2008-03
3 JI Yan-qing et al(College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018);Movement and Distribution of Anions in Cucumber Greenhouse Soil[J];Journal of Anhui Agricultural Sciences;2007-02
4 ZHANG De-kui et al (Gansu Key Laboratory of Desertification Combating/Gansu Desert Control Research Institute,Wuwei,Gansu 733000);Application of the Fuzzy Resemble Priority Ratio in the Study on Endurance of Tamarix L. to Salt[J];Journal of Anhui Agricultural Sciences;2007-12
5 MA Yong-gui et al(College of Life and Geography Science,Qinghai Normal University,Xining,Qinghai 810008);Study on Contents of Pro and ABA in Two Plant Leaves in the Bank of Qinghai Lake[J];Journal of Anhui Agricultural Sciences;2007-23
6 XU Da-yong et al(Institute of Aquatic Environment Integrated Rehabilitation,College of Environmental Science and Engineering of Tongji University,Shanghai 200092);Research on the Eco-physiology Characteristic and the Pollutant Removal Mechanism of Artificial Wetland Macrophyte[J];Journal of Anhui Agricultural Sciences;2008-03
7 MA Hong-ying et al(The New and High Agricultural Technology Demonstration Gardens of Tianjin City,Tianjin 300384);Study on Salinity Tolerance of Tomatoes during Seed Germination under Different Salt Stress Conditions[J];Journal of Anhui Agricultural Sciences;2008-32
8 ZHOU Chao-bin et al(College of Agriculture,Shihezi University,Shihezi,Xinjiang 832000);Further Studies on Populus euphratica Adaptation Mechanism and Resistance to Drought[J];Journal of Anhui Agricultural Sciences;2009-03
9 Huang Wenchuang et al (Hefei Institute of Economics and Technology,Hefei 230052);The Influence of Salt Stress on Tobacco Seed Germination[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1999-02
10 SI Jian-hua, FENG Qi, ZHANG Xiao-you (Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou Gansu 730000, China);Application of Heat-pulse Technique to Determine the Stem Sap Flow of Populus euphratica[J];Journal of Glaciology and Geocryology;2004-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiao-yan,XU Kun*(College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China);Effect of Rootstock-scion Interaction on Antioxidant Enzyme System in Leaves of Grafted Eggplant Seedlings under Low Temperature Stress[J];Shandong Agricultural Sciences;2009-06
2 WANG Pei et al (Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining,Qinghai 810001);Cell Localization of Atfin1 Zinc Finger Protein in Arabidopsis[J];Journal of Anhui Agricultural Sciences;2008-19
3 ZHOU Chao-bin et al(College of Agriculture,Shihezi University,Shihezi,Xinjiang 832000);Further Studies on Populus euphratica Adaptation Mechanism and Resistance to Drought[J];Journal of Anhui Agricultural Sciences;2009-03
4 LIU Gui-lin1,2,Alishir KURBAN1,2,Arkin ABAYDULLA3,DUAN Han-ming4, Umut HALIK5,Abdimijit ABLEKIM1,NIU Ting1,2(1.Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,ürümqi Xinjiang 830011,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.Xinjiang Tarim River Basin Management Bureau,Korla Xinjiang 841000,China;4.China West Normal University,Nanchong Sichuan 637002,China;5.College of Resource and Environmental Sciences/Key Laboratory of Oasis Ecology of Xinjiang,Xinjiang University,ürümqi Xinjiang 830046,China);Changes in Landscape Pattern in the Lower Reaches of Tarim River after an Ecological Water Delivery[J];Journal of Glaciology and Geocryology;2012-01
5 LI Zhen-zhen1,TIAN Li-ping2,ZHANG Tian-yi1,PAN Cun-e1,ZHANG Hai-li1(1.College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832000;2.College of Pharmacy,Shihezi University,Shihezi,Xinjiang 832000);Study on Dynamic Changes of Soil Moisture and Salt of Artificial Populus euphratica Forest in Summer[J];Northern Horticulture;2010-22
6 HAN Lu~ 1,2 ;WANG Hai-zhen~ 1,2 ;PENG Jie~1;LIANG Ji-ye~1;MA Chun-hui~2.1 College of Plant Science and Technology,Tarim University,Alar,Xinjiang,843300,P. R.China;2 Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Xinjiang Production & Construction Corps,Alar,843300,P. R.China.;Size-class structure and distribution pattern of Populus euphratica Oliv.in different habitats[J];Journal of Beijing Forestry University;2010-01
7 HAO Jian-qing;L Na;YANG Yang;LI Wen-hai;BAI Xue;ZHENG Cai-xia National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,College of Biological Sciences and Biotechnology,Beijing Forestry University,100083,P.R.China.;Comparative study of chlorophyll fluorescence parameters and water physiological characters of heteromorphic leaves for Populus euphratica[J];Journal of Beijing Forestry University;2010-05
8 LIU Pu-xing1;LU Chen-yu1;YAO Xiao-jun1,2;CAO Li-guo1. 1 College of Geography and Environment Science,Northwest Normal University,Lanzhou,730070,P.R.China;2 Cold and Arid Region Environment and Engineering Research Institute,Lanzhou,730000,P.R.China.;Structure and spatial distribution patterns of Populus euphratica populations from different habitats in the Dunhuang Oasis[J];Journal of Beijing Forestry University;2011-02
9 SHI Jun-na;LIU Mei-qin;SHI Jing;WANG Yan-ping;ZHI Guan-hua;CHEN Yu-zhen;LU Cun-fu.National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing Forestry University,100083,P.R.China.;Sequence analysis and expression pattern of AmZFPG encoding a GATA type zinc finger protein in Ammopiptanthus mongolicus[J];Journal of Beijing Forestry University;2011-03
10 JIANG Xi-bing1;SONG Yue-peng1;MA Kai-feng1;GUO Bin1;AN Xin-min1;ZHANG Zhi-yi1;SHI Zhi-wei2;XU Lan-li2;ZHANG You-hui3.1 National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,Beijing Forestry University,100083,P.R.China;2 Forest Administration of Pingquan County,Hebei,067500,P.R.China;3 National Nursery of Guanxian County,Shandong,252500,P.R.China.;Changes of several physiological indices in hybrid clones of Populus deltoides Bartr.×P.ussuriensis Kom.under low temperature stress[J];Journal of Beijing Forestry University;2012-01
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 HAO Lu-Ning and YU Shu-Wen (Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032);Preparation of Sealed Plasma Membrane Vesicles from Barley Roots and Their Activities in H~+ and Ca~(2+) Transport[J];;1992-04
2 Zhu Mei jun and Chen Jia (College of Biological Sciences, Beijing Agricultural University, Beijing 100094);SUBCELLULAR LOCALIZATION AND KINETIC CHARACTERIZATION OF ABA BINDING PROTEINS IN MAIZE ROOT[J];Acta Botanica Sinica;1995-12
3 GU Rui-Sheng;JIANG Xiang-Ning;GUO zhong-Chen(Experimental Center of Forest Biology, Beijing Forestry University, Beijing 100083)(Institute of Botany of Botany,The Chinese Academy of Sciences, Beijing 100093);Organogenesis and Plantlet Regeneration in Vitro of Populus euphratica[J];ACTA BOTANICA SINICA;1999-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved