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《Acta Botanica Sinica》 2001-12
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Expression of Human Epidermal Growth Factor Gene in Cyanobacteria

DAI Wei 1.2, SHI Ding-Ji 1 *, ZHANG Hui 1,2, ZHONG Hui 3, RAN Liang 1, PENG Guo-Hong 4, GAN Ren-Bao 5, CHEN Su-Juan 3, LIAN Mu-Lan 2(1. Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; 2. Department of Biol  
The human epidermal growth factor (hEGF) is a small single-chain polypeptide of 53 amino acid residues. It can stimulate the proliferation of many cell types, mainly those of epidermal and epithelial tissues both in vivo and in vitro. A vector pRL-hEGF was constructed using plasmids pRL-489 and pUC-hEGF. The synthetic hEGF gene was recombined into the downstream of strong promoter psbA in plasmids pRL-489. Then, the vector was introduced into Synechococcus sp. PCC 7002 and Anabaena sp. PCC 7120 by triparental conjugative transfer. The transformation was confirmed by PCR amplification. The pRL-hEGF is thought to be retained as a plasmid form in the transgenic Anabaena sp. PCC 7120, since it can be recovered. However, it has been integrated into the chromosome of Synechococcussp. PCC 7002 as there is no duplication origin in the pRL-hEGF in this cyanobacterium and plasmid cannot be isolated from the Synechococcussp. PCC 7002 either. The radioimmunoassay (RIA) proved that the hEGF gene has been expressed as the protein existed in these two strains of transgenic cyanobacteria, and the hEGF protein in Anabaenasp. PCC 7002 could be secreted into the medium.
【Fund】: 国家“海洋 86 3”高技术计划基金 (819-0 4-11)~~
【CateGory Index】: Q943.2
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