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《植物学报(英文版)》 2002-10
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Cloning and Characterization of the Na~+/H~+ Antiport Genes from Triticum aestivum

WANG Zi-Ning 1,2, ZHANG Jin-Song1, GUO Bei-Hai2, HE Si-Jie1, TIAN Ai-Guo1, CHEN Shou-Yi1* (1.Plant Biotechnology Laboratory, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China; 2.Plant Breeding Laboratory, Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050031, China)  
The Na+/H+ antiport genes namedTaNHX1andTaNHX2were cloned by screening a salt_stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed thatTaNHX1was 2 029 bp in length and contained a complete ORF of 1 638 bp. TheTaNHX1encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI.TaNHX2was 1 693 bp in length consisting of a partial ORF followed by a 3′_UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. A RT_PCR assay showed that the level ofTaNHX1transcripts was increased and reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.
【Fund】: 国家转基因专项资助项目 (J99-A-0 0 4 J00-A-00 8-0 2 );; 国家重点基础研究发展规划项目 (G19990 1170 0 3 )。~~
【CateGory Index】: Q943
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