Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《植物学报(英文版)》 2002-10
Add to Favorite Get Latest Update

Cloning and Characterization of the Na~+/H~+ Antiport Genes from Triticum aestivum

WANG Zi-Ning 1,2, ZHANG Jin-Song1, GUO Bei-Hai2, HE Si-Jie1, TIAN Ai-Guo1, CHEN Shou-Yi1* (1.Plant Biotechnology Laboratory, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China; 2.Plant Breeding Laboratory, Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050031, China)  
The Na+/H+ antiport genes namedTaNHX1andTaNHX2were cloned by screening a salt_stressed wheat cDNA library using rice Na+/H+ antiport cDNA fragment as the probe. Sequencing analysis showed thatTaNHX1was 2 029 bp in length and contained a complete ORF of 1 638 bp. TheTaNHX1encodes a polypeptide of 546 amino acids with a transmembrane domain DIFFIYLLPPI.TaNHX2was 1 693 bp in length consisting of a partial ORF followed by a 3′_UTR of 808 bp. The amino acid sequence of these two genes were about 70% identical to the known NHX genes from rice, Arabidopsis and Atriplex. A RT_PCR assay showed that the level ofTaNHX1transcripts was increased and reached a steady higher level in the seedlings after 3 h treatment with 400 mmol/L NaCl.
【Fund】: 国家转基因专项资助项目 (J99-A-0 0 4 J00-A-00 8-0 2 );; 国家重点基础研究发展规划项目 (G19990 1170 0 3 )。~~
【CateGory Index】: Q943
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jun-lian~(1,2),ZHANG Jin-wen~(1,2),CHEN Zheng-hua~3,WANG Di~(1,2)(1.Agronomic College of Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement,Lanzhou 730070,China;3.Postdoctor Workstation of Yasheng Industrial Ltd.,Beijing 100101,China);Cloning and sequence analysis of a cDNA encoding Na~+/H~+ antiporter(AtNHX1) from Arabidopsis thalian under NaCl stress[J];Acta Pratacultural Science;2005-06
2 Wang Lei(College of Life Sciences,Northeast Agricultural University,Harbin 150030,P.R.China);Xu Dechang(Harbin Institute of Technology);Zhu Yanming,Bai Xi(Northeast Agricultural University);Ion Homeostasis Regulatory Genes and Their Application to Genetic Engineering of Salinity Tolerance in Plants[J];Journal of Northeast Forestry University;2008-06
3 LAN Tao1,2,DUAN Yuan-lin1,2,WU Wei-ren1,2,WANG Bin3,ZHOU Yuan-chang1,LI Sheng-ping3,DIAO Zhi-juan3(1.College of Crop Science,Fujian Agriculture and Forestry University;2.Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding;3.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Cloning and sequence analysis of 3′cDNA end of Na~+/H~+ antiporter gene from Spartina anglica[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2007-02
4 SUN Jian-chang1,2,WANG Xing-sheng2,YANG Sheng-long2 (1.Northwest A & F University,Yangling,Shaanxi 712100,China; 2.Ningxia Academy of Agriculture and Forestry Sciences,Yongning,Ningxia 750105,China);Progress of research on salt-resistance in plants[J];Agricultural Research in the Arid Areas;2008-01
5 CUI Run-li1,2,ZHI Hui1,WANG Yong-fang1,LI Wei1,LI Hai-quan1,HUANG Zhan-jing3,DIAO Xian-min1(1.National Millet Improvement Center,Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,China;2.Hebei Chemical and Pharmaceutical College,Shijiazhuang 050016,China;3.College of Life Sciences,Hebei Normal University,Shijiazhuang 050016,China);Cloning of DnaJ-like Protein Gene from Foxtail Millet[J];Acta Agriculturae Boreali-Sinica;2007-04
6 QIAN Yong-sheng,ZHANG Xiao-qin(School of Life and Environment Science,Hangzhou Normal College,Hangzhou 310036,China);Progress on Na~+/H~+ Antiporter in Plants under Salt Stress[J];Hubei Agricultural Sciences;2007-02
7 CUI Run-li~2 DIAO Xian-min~1,2 (1 The National Millet Improvement Center, Institute of Millet, Hebei Academy of Agriculture and Forest Shijiazhuang 050031, China) (2 College of Life Science, Hebei Normal University Shijiazhuang 050016, China );Advances on Cloning and Translation of the Salt Tolerance Genes in Plant[J];Progress In Biotechnology;2005-08
8 ZHANG Geng, WANG Zan, GUAN Ning, WANG Xue-Min, LI Yuan, GAO Hong-Wen Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China;Molecular cloning and bioinformatics analysis of a vacuolar Na~+/H~+ antiporter gene in Elytrigia intermedia[J];Hereditas;2007-10
9 LI Hui,LI De-fang,CHEN An-guo,TANG Hui-juan,BAI Feng-hu(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha Hunan 410205,China);Progress on Research of Salt-tolerant Plants[J];Plant Fiber Sciences in China;2007-04
10 ZENG Hua-zong,LUO Li-jun (Shanghai Agrobiological Gene Center,Shanghai 201106);A Review on Plant Drought and Salt Tolerance Gene[J];Journal of Plant Genetic Resources;2003-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YAO Fang-yin,ZHOU Xue-biao & LIU Li-mei (Shandong Rice Research Institute,Jining 272007);Rice germplasm with bacterial blight resistance obtained by introducing foreign total DNA[J];SHANDONG AGRICULTURAL SCIENCES;2000-01
2 Shao Hongyu~1 Kong Guangchao~1 Qi Juncang~1 Ren Litong~2 Wang Ruiqing~(1,3) Cao Lianpu~1(1 Agriculture College of Shihezi University/ Key laboratory of Oasis Ecology Agriculture of Xinjiang Crops 832000;2 Chemical College of Shihezi University 832000;3 Institute of Science and Technology,Tarim University 843300);Advances in research on physiological and biochemical characteristics of salt tolerance in plant[J];Anhui Agricultural Science Bulletin;2006-09
3 Quan Xianqian (Department of Biology, Linyi Normal University, Linyi, S handong 276005);Enzymatic Mechanism of Scavenging Reactive Oxygen Species of Euh alophytes[J];Journal of Anhui Agricultural Sciences;2003-02
4 Quan Xianqing (Department of Biology, Linyi Normal University, Linyi,Shandong 276005);No-enzymatic Mechanism of Scavenging Reactive Oxygen Species of Euhalophytes[J];Journal of Anhui Agricultural Sciences;2003-03
5 SHEN Yu-xiang et al (Department of Chemistry and Biotechnology,Yancheng Technology College,Yancheng,Jiangsu 224002);Effects of Salt Stress on the Population Quality,Yield and Protein Content in Wheat Cultivar Ningyan 1[J];Journal of Anhui Agricultural Sciences;2008-34
6 CHANG Hong-jun~1,QIN Yu-qian~2 (1.Anyang Normal University,Anyang 455000,China;2.Nanyang Institute of Technology,Nanyang 473000,China);The Physiology of Plant's Salt Stress[J];Journal of Anyang Teachers College;2006-05
7 ZHEN Wei WANG Yangming\ DING Wei\ CHEN Xi\ Hu Yuanlei\ LIN Zhongping (National Laboratory of Protein Engineering and Plant Genetic Engineering,Peking University,Beijing,100871);Cre/loxP-mediated Deletion of Transgene in Transgenic Tobacco[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2001-04
8 Wang Yanqing;Chen Xuemei;Li Yue;Jiang Xiangning;Liu Qunlu;Li Suyan. College of Biology, Beijing For. Univ., 100083, P. R. China.;The osmotic solute in plant resistance to adverse conditions and progress in relative genic engineering.[J];Journal of Beijing Forestry University;2001-04
9 LU Duan zheng (Herbarium of Forestry Plants in Beijing,Beijing 100083);New Materal of the Plants of Beijing[J];Journal of Beijing Agricultural College;2002-03
10 AN Jian~1,WANG Ming~(2*),WANG Li-xia~1,YANG Jing~3,XIA Mao-hua~3(1.Beijing Agricultural College, Beijing 102206; 2.China Agricultural University, Beijing 100094;Beijing ZOO,Beijing 100044);mRNA Differential Display and Its Application on Drug-Resistance of Coccidia[J];Journal of Beijing Agricultural College;2005-02
China Proceedings of conference Full-text Database 1 Hits
1 Guo Tiancai (National Engineering Research Centre for Wheat);Chinese wheat production and development suggestions[A];[C];2002
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 DUAN Dai-xiang(Department of Life Sciences,Binzhou University of Shandong,Binzhou,Shandong 256603);Research Progress on Salinity Resistance of Salicornia bigelovii[J];Journal of Anhui Agricultural Sciences;2009-23
2 LIU Gen-hong,XIE Ying-zhong,LAN Jian,YANG Rui,ZHAO Gong-qiang(Ningxia University,Yinchuan,Ningxia 750021,China);Chlorophyll Content Trends During Seedling Stage of Ten Staple Alfalfa Varieties in Ningxia with NaCl and Water Compound Stress[J];Northern Horticulture;2008-10
3 LIU Tai-lin1,YANG Jing-hui1,LIU Yan-jun1,ZHANG Wei-yu2(1.Department of Horticulture,Tianjin Agricultural College,Tianjin 300384,China;2.Department of Mechanical and Electrical Engineering,Tianjin Agricultural College,Tianjin 300384,China);The PUTNHX Gene Transformation and The Salt-tolerance Characteristic of Transgenic Plants in Melilotus suavena[J];Northern Horticulture;2009-11
4 MA Chao-ying,LI Xiao-liu.SHI Hong-ling,CHEN Chao,CUI Xue-zheng (Department of Life Science.Tangshan Teachers College,Tangshan,Hebei 063000);The Common Species and Application of Salt-tolerant Plants[J];Northern Horticulture;2010-03
5 WANG Ying1,JIN Hua2,LIU Ming-guo1,YAN Yan-hua3,JIANG Guo-bin2(1.College of Forestry,Shenyang Agriculture University,Shenyang,Liaoning 110161;2.College of Life Sciences,Dalian National University,Dalian,Liaoning 116600;3.College of Life Sciences,Liaoning Normal University,Dalian,Liaoning 116029);Genetic Engineering of Biological Stress Tolerance in Poplar[J];Northern Horticulture;2010-24
6 LIU Yu-yan1,YU Feng-ming2,CAO Hui-ying1,ZHANG Li-juan3,DAI Bo1(1.College of Horticulture and Technology,Hebei Normal University of Science and Technology,Changli,Hebei 066600;2.College of Life and Technology,Hebei Normal University of Science and Technology,Changli,Hebei 066600;3.Hebei Tourism Vocational College,Chengde,Hebei 067000);Effects of Salt Stress on Seed Germination of Volia yedoensis Makino[J];Northern Horticulture;2011-05
7 LIU Jin-rong1,2,SUN Ji-xiong1,XIE Xiao-rong1,2,DU Jian-xiong1,3,WANG Qi1(1.Pratacultural College,Gansu Agricultural University,Lanzhou, Gansu Province 730070,China;2.Key Laboratory of Western China Resource and Environmental Chemistry,Department of Biology,Hexi University,Zhangye,Gansu Province 734000,China;3.School of Resources and Environmental Management,Guizhou College of Finance and Economics,Guiyang,Guizhou Province 550004,China);Effect of Understratum-Film on Saline Partition and Establishment of High Quality Turf in Heavy Saline-Alkali Field of Arid Hungriness Oasis Region[J];Acta Agrestia Sinica;2008-02
8 ZHAO Rui-xue,ZHU Hui-sen,CHENG Yu-hong,DONG Kuan-hu (College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China);Research progress on proline and its biosynthesis enzymes in plant[J];Pratacultural Science;2008-02
9 Li Jian,Wang Youju,Jiang Jing,Liu Guifeng,Wang Lei(Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education(Northeast Forestry University),Harbin 150040,P.R.China);Yang Lianhe(Kuangzhu Forestry Farm of Benxi City,Liaoning Province);Expression of eIF1A Gene in Transgenic Populus davidiana×P.bolleana and Its Salt Tolerance[J];Journal of Northeast Forestry University;2010-01
10 Hao Fengge,Zhou Junguo,Zhou Xiumei(College of Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,P.R.China);Effects of NaCl Stress on Leaf Physiological Characteristics and Growth of Camptotheca acuminata Seedlings[J];Journal of Northeast Forestry University;2010-01
China Proceedings of conference Full-text Database 2 Hits
1 DONG Li-hua LAN Jian YAO Ai-xing (pratuacultural Science Institute of Ningxia University, Yinchuan 750021, China);Review on Salinity and Drought Tolerance of Turfgrass[A];[C];2004
2 LAN Jian~(1,2) XIE Yingzhong~(1,2) LIU Genhong~1 YANG Rui~1 ZHAO Gongqiang~1 (1.Grassland Institute Ningxia University Yinchuan 750021) (2.Key Laboratory of Degraded Ecosystem Restoration and Rehabilitation in Northwest China Ministry of Education Ningxia University Yinchuan 750021);Comprehensive Evaluation about Alfalfa Seed Germination Resistance to Drought and Salt Stress[A];[C];2007
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved