STUDIES OF SIGNAL TRANSDUCTION OF SALICYLIC ACID IN CUCUMBER CELLS
1LI ZhaoLiang 2YUAN YongBing 2LIU ChengLian 1CAO ZongXun (Tsao T H)1(Department of Plant Molecular and Developmental Biology,College of Life Sciences, Peking University, Beijing 100871)2(Department of Horticulture,
For the first time, the signal transduction pathway of salicylic acid (SA) was investigated by using 3Hlabelling, thinlayer chromatography and anion exchange column chromatography. It was found that SA stimulated the activity of membrane bound phospholipase C (PLC), accelerated the breakdown of phosphatidylinositol4monophosphate (PIP) and phosphatidylinositol4,5bisphosphate (PIP2) and increased the levels of inositol1,4bisphosphate (IP2),inositol1, 4,5trisphosphate (IP3) and diacylglycerol (DAG). These indicated that signal transduction of SA was probably accomplished through the mediation of phosphatidylinositide signal transduction system in cucumber(Cucumis sativa L.).