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《Journal of Chinese Microcirculation》 2003-04
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An Research on Expression of IL-8and Attraction to PMN of PMVEC Directly-Induced by LPS

GU Da-yong,LI Yan,CHEN Li,et al.Clinical Experimental Institute of Chendu Army General Hospital,Chendu610083,China  
Objective To study the changes of pulmonary microvascular endothelial cells'(PMVEC)ex-pression of IL-8and its attraction to PMN directly induced by LPS.Methods With methods of ISH,ELISA re-spectively to examine the expressions of IL-8mRNA in PMVEC and its protein secretion in the supernatant of culture medium,when PMVEC is directly excited by100ng/ml LPS at0h,1h,2h,4h,6h,8h or1ng/ml,10ng/ml,100ng/ml LPS at6h.At the same time,to examine the attraction index of PMVEC to PMN with the agarose plate assay and the inhibited effects on it by using anti-IL-8antibody.Results LPS could directly act on the PMVEC to promote the IL-8expression,including promoting the expression of IL-8mRNA and secretion of IL-8pro-tein,and the mRNA expressions increased before the protein secreted in terms of time.The LPS excitement could also significantly promote the attraction of PMVEC to PMN,while anti-IL-8antibody could significantly restrain this attraction.Conclusions The directly-induction of LPS could promote PMVEC to the expression and secretion of IL-8.So providing the required physical condition for the PMN migration,and then intensifying the attraction to PMN.It could play an important role in inflammatory response induced by LPS.
【CateGory Index】: R363
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