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《Journal of Chinese Microcirculation》 2006-01
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A Research on Expression of ICAM-1 and Adhesion to PMN of PMVEC Directly-Induced by LPS

GU Da-yong,LIANG Bing,TANG Xu-dong,et al.Science Laboratory,Number 401 Hospital of PLA,Shandong Qingdao 266071,China. Clinical Experimental Institute,Chendu Army General Hospital of PLA,Sichuan Chendu 610083,China  
Objective To study the changes of pulmonary microvascular endothelial cells'(PMVEC) expression of ICAM-1 and its adhesion to PMN directly induced by LPS.Methods When PMVEC was directly excited by 100ng/ml LPS at 0h,1h,2h,4h,6h,8h or 1ng/ml,10ng/ml,100ng/mlLPS at 6h,the expressions of ICAM-1 in PMVEC were examined with ICC.At the same time,the adhesion rates of PMVEC to PMN and the inhibited effects on it by using Anti-ICAM-1 antibody were examined.PMVEC-PMN's adhesion was observed in a static state using scanning electron-microscope. Results The ICAM-1 expressions increased with the time and dosage increasing of LPS direct excitement.LPS could directly induce the adhesion increase of PMVEC to PMN at almost the same time and in almost the same mode as ICAM-1 expressions.Anti-ICAM-1 anti-body could restrain significantly the PMVEC-PMN adhesion induced by LPS(P0.01).Scanning electron-microscope directly showed PMVEC-PMN adhesion as well as the existence of the secondary tethering phenomenon.Conclusion The direct-induction of LPS could promote PMVEC to express ICAM-1,hence provided physical basis for the adhesion of PMVEC to PMN.The occurrence of the "secondary tethering" further expands and consolidates the adhesion.
【CateGory Index】: R363
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