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《Journal of Chinese Microcirculation》 2006-01
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A Research on Expression of ICAM-1 and Adhesion to PMN of PMVEC Directly-Induced by LPS

GU Da-yong,LIANG Bing,TANG Xu-dong,et al.Science Laboratory,Number 401 Hospital of PLA,Shandong Qingdao 266071,China. Clinical Experimental Institute,Chendu Army General Hospital of PLA,Sichuan Chendu 610083,China  
Objective To study the changes of pulmonary microvascular endothelial cells'(PMVEC) expression of ICAM-1 and its adhesion to PMN directly induced by LPS.Methods When PMVEC was directly excited by 100ng/ml LPS at 0h,1h,2h,4h,6h,8h or 1ng/ml,10ng/ml,100ng/mlLPS at 6h,the expressions of ICAM-1 in PMVEC were examined with ICC.At the same time,the adhesion rates of PMVEC to PMN and the inhibited effects on it by using Anti-ICAM-1 antibody were examined.PMVEC-PMN's adhesion was observed in a static state using scanning electron-microscope. Results The ICAM-1 expressions increased with the time and dosage increasing of LPS direct excitement.LPS could directly induce the adhesion increase of PMVEC to PMN at almost the same time and in almost the same mode as ICAM-1 expressions.Anti-ICAM-1 anti-body could restrain significantly the PMVEC-PMN adhesion induced by LPS(P0.01).Scanning electron-microscope directly showed PMVEC-PMN adhesion as well as the existence of the secondary tethering phenomenon.Conclusion The direct-induction of LPS could promote PMVEC to express ICAM-1,hence provided physical basis for the adhesion of PMVEC to PMN.The occurrence of the "secondary tethering" further expands and consolidates the adhesion.
【CateGory Index】: R363
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Chinese Journal Full-text Database 4 Hits
1 ZHAO Shuangping,WU Jiao,GUO Qulian,ZHANG Zhong,YE Zhi(Intensive Care Unit,Xiangya Hospital,Central South University,Changsha 410008,China);Effect of different concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats[J];Journal of Central South University(Medical Science);2010-09
2 LUO Xing,MIAO Chang-hong,GE Bao-xue,JIANG Hao(Department of Anesthesiology and Critical Care Medicine,Fudan University,Zhongshan Hospital,Shanghai 200032,China);Effects of isoflurane,sevoflurane and desflurane on expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells[J];Journal of Zhejiang University(Medical Sciences);2010-05
3 YAO Shu-tong,ZHAO Xiu-mei,LIU Xiu-hua.Dept.of Pathophysiology,PLA General Hospital,Beijing 100853,China;ICAM-1 Inhibition Involving Small Intestinal Protection Induced by Ischemic Postconditioning in Rats[J];Journal of Chinese Microcirculation;2008-02
4 WU Yuan,JING Li-ming,CHEN Xiao-wen,HE hui-juan.Department of Microbiology and Immunology,Guangdong Medical College;Institute of Nephrology,Affiliated Hospital to Guangdong Medical College,Zhanjiang 524001,China;Study on Tumor Necrosis Factor-α and Lipopolysaccharides Inducing Interleukin-12 Expression in Mesangial Cells[J];Journal of Chinese Microcirculation;2008-02
Chinese Journal Full-text Database 1 Hits
1 GU Da_yong,ZENG Xiang_yuan,CHEN Li,et al. Clinical Experintal Institute of Chendu Army General Hospital 610083, China;An Experimental Study on ICAM-1 Expression of Pulmonary Microvascular Endothelial Cells Induced by LPS and Its Mechanism Through Nuclear Factor-kappa B Pathway[J];Journal of Chinese Microcirculation;2002-01
Chinese Journal Full-text Database 10 Hits
1 WANG Zi-li,SUO Zhan-wei,MU Xiang,XU Jian-qin(College of Animal Medicine, China Agricultural University,Beijing,100094,China);Advancement of Effects of Endotoxin on the Microcirculation and the Functions of Vascular Endothelial Cells[J];Progress In Veterinary Medicine;2003-06
2 ;Immunologic Mechanisms in the Inflammatory Response to Cerebral Ischemic-Reperfusion Injury[J];Anatomy and Clinics;2007-06
3 Gu Dayong 1,Wang Hua 1,Liu Jinbiao 1,et al.1.Moleuclar Biology Center,Research Institute of Surgery,The Third Military Medical University,Chongqing 400000,China;2.Clinical Experimental Institute,Chengdu Army General Hospital,Chengdu 610083,China;An research on the expression of IL-8 in PMVEC directly-induced by LPS and its mechanism through the Nuclear Factor-Kappa B[J];Sichuan Medical Journal;2004-09
4 Zhu Weiguo,Jin Yanhua,Bai Xiaohong,et al.The Second People's Hospital of Liangshan,Xichang Sichuan 615000,China;An research on the expression of IL-8 in PMVEC and its mechanism through the Nuclear Factor-Kappa B[J];Sichuan Medical Journal;2005-12
5 FENG Guo-ji,GU Da-yong,CHEN Li, MA Bu-ren, YANG Ji-yun,ZENG Xiang-yuan (No. 401 Hospital of PLA,Shandong Qingdao 266071 .China; Research Institute of Surgery Raping Hospital,the Third Military Medical University, Chongqing 400042 China; Institute of Clinical Laboratory, Chengdu General Hospital of Chengdu Command of PLA,Sichuan Chengdu 610083,China);Research on the Expression of IL-8 in PMVEC Directly-Induced by LPS and Its Mechanism through the Nuclear Factor-kappa B[J];Shaaxi Journal of Medical Laboratory Sciences;2003-04
6 Ding Xing,Zhan Zhen;Research on the Relationship Between Commen Changes of Coating of Tongue and the Expression Level of ICAM-1[J];Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine;2007-03
7 HUANG Qi - ren, CHEN He - ping, LIU Dan, ZENG Guo - hua, HE Ming ( Department of Pharmacology, Jiangxi Medical College, Nanchang 330006, China);Role of MEK1/2 in ICAM -1 over expression induced by lipopolysaccharide in human umbilical vein endothelial cells[J];Chinese Journal of Pahophysiology;2005-12
8 LIU Gang,FU Ai-jun,ZHU Jun,et al.Neurosurgical Department of Coal Medical College of North China,Tangshan 063000,China;Dynamic observation of pathological change and activation of nuclear factor-kappa B in the intestinal mucosa of rats after diffuse brain injury[J];Chinese Journal of Critical Care Medicine;2005-12
9 GU Da-yong,LI Yan,CHEN Li,et al.Clinical Experimental Institute of Chendu Army General Hospital,Chendu610083,China;An Research on Expression of IL-8and Attraction to PMN of PMVEC Directly-Induced by LPS[J];Journal of Chinese Microcirculation;2003-04
10 GU HAI-feng,NI Jun,ZHANG Xing-mei. Department of Plastic Surgery,PLA No.85 Hospital,Shanghai 200052,China;Effects of Microfilaments on the Repair of Wounded Endothelial Monolayers[J];Journal of Chinese Microcirculation;2006-05
Chinese Journal Full-text Database 10 Hits
1 LI Yu-mei, CHEN Xu-hua (The First Department of Clinical Medicine,Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China);Effect of Danshen on L-type Calcium Channel Current in Isolated Myocardiocytes After Ischemia[J];Journal of Anhui Traditional Chinese Medical College;2003-04
2 QU Shao-chun1,SUI Cheng1,2,YU Xiao-feng1,WANG Xuan-hui1,HE Xiao-xi,XU Hua-li1(1.Department of Pharmacology,School of Pharmacy,Jilin University,Changchun 130021,China;2.Department of Pharmaceutics,School of Pharmacy,China Pharmaceutical University,Nanjing 210038,China);Protective effects of Ginsenoside-Rb on experimental myocardial ischemia-reperfusion injury in rats[J];Journal of Jilin University(Medicine Edition);2007-05
3 GENG Bin1, DU Jun-bao2, TANG Chao-shu 1△(1.Institute of Cardiovascular Research, 2.Department of Pediatrics,Peking University First Hospital, Beijing 100034,China);Application of sensitive sulphur electrode assay to measure and analyze cystathionine-γ-lyase/hydrogen sulfide pathway of cardiovascular tissues, cells and plasma in rats[J];Journal of Peking University(Health Sciences);2005-05
4 LIU Xiuhua;PANG Yongzheng;SU Jingyi;TANG Chaoshu(Laboratory of Shock Pathophysiology,Institute of Cardiovascular Research,Beijing Medical University 100083);PRECONDITIONING PROTECTES INTESTINE FROM ISCHEMIC REPERFUSION INJURY OF RAT[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1996-01
5 WANG Chunyan, ZHAO Zhongxin, XIA Bin Department of Neurology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Expressions of ICAM-1 and VCAM-1 associated with ischemia-reperfusion brain injury[J];Chinese Journal of Neuromedicine;2003-06
6 Kazuyoshi Kuwano ,Division of Respiratory Diseases,Department of Internal Medicine,Jikei University School of Medicine,Tokyo,Japan;;Epithelial Cell Apoptosis and Lung Remodeling[J];中国免疫学杂志(英文版);2007-06
7 DENG You-hua,GUO Zheng-heng,CHEN Hong-ming,et al.Department of Cardiothoracic Surgery, YouJiang National Medical College,Baise,533000,China;Impacts of Anisodamine(654-2) Combined with Exogenous Pulmonary Surfactant on Tomor Necrosis Factor (TNF-α),Structure Changes of Lung Tissue During Pig's Lung Ischemic-reperfusion Injury[J];Journal of Chinese Physician;2002-04
8 LI Qi, QIAN Gui sheng, ZHANG Qing, WANG Chang zheng (Institute of Respiratory Diseases, Xingqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of different doses of lipopolysaccharide on acute lung injury in rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-10
9 GU Xiao-ming1,HE Ming2 (1.LongHua Epidemic-Prevention and Healthcare Institute of Baoan District,Shenzhen,Guangdong 5181091,China;2.Department of Pharmacology,Medical College of NanChang University,Nanchang,Jiangxi 330006,China);Delayed effects of TMPZ on neonatal rat myocardiocytes subjected to anoxia/reoxygenation[J];Journal of Guangdong College of Pharmacy;2007-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YAO Shu-tong,SHANG Zhan-ping,SANG Hui,FANG Yong-qi,YU Feng-xiu,WANG Jia-fu(Dept.of Pathophysiology,Taishan Medical College,Taian 271000,China);Protective effect of ischemic postconditioning on lipid peroxidation of lung following intestinal ischemia reperfusion in rats[J];Journal of Taishan Medical College;2010-03
2 Zhao Xia,Dong Shuyun,Zhang Lianyuan North China Coal Medical college,Tangshan 063000;The Changes of Mesentery Microcirculation in Rats with Hind Limbs Ischemia-Reperfusion[J];Chinese Journal of Microcirculation;2010-01
3 WANG Lei,Mamatzunun·Mansur,Kelala·Abasi,et al(Department of Pathophysiology,Preclinical Medical College,Xinjiang Medical University,Urumqi 830011,China);Protection effects of grape-seed proanthocyanidins extrac on renal ischemia reperfusion injury in rats[J];Journal of Xinjiang Medical University;2011-01
4 YAO Shu-tong,SANG Hui,SHANG Zhan-ping,TIAN Hua,FANG Yong-qi,WANG Jia-fu(Department of Pathophysiology,1Institute of Atherosclerosis,Taishan Medical College,Taian 271000,China);EFFECTS OF WATER EXTRACT OF PROPOLIS ON LUNG INJURY AND NF-κB/ICAM-1 PATHWAY IN LUNG FOLLOWING INTESTINAL ISCHEMIA REPERFUSION IN RATS[J];Acta Nutrimenta Sinica;2011-01
5 YAO Shu-tong1,2,LIU Xiu-hua1,TANG Xu-ming1,SUN Sheng1,WANG Jia-fu2(1Department of Pathophysiology,Chinese PLA General Hospital,Beijing 100853,China; 2Department of Pathophysiology,Taishan Medical College,Taian 271000,China.;Ischemic postconditioning ameliorates pia mater microcirculation in rats subjected to cerebral ischemia reperfusion[J];Chinese Journal of Pathophysiology;2009-03
6 YAO Shu-tong,SANG Hui,WANG Jia-fu,SHANG Zhan-ping,LI Wei-hong,YU Feng-xiu(Department of Pathophysiology,Taishan Medical College,Taian 271000,China. );Ameliorative effect of water extract of propolis preconditioning on mesenteric microcirculation of rats subjected to small intestinal ischemia reperfusion[J];Chinese Journal of Pathophysiology;2010-01
7 YAO Shu-tong1,SANG Hui1,SHANG Zhan-ping1,TIAN Hua2,FANG Yong-qi1,YU Feng-xiu1,WANG Jia-fu1(1Department of Pathophysiology,2Institute of Atherosclerosis,Taishan Medical College,Taian 271000,China.);Ischemic postconditioning inhibits activation of nuclear factor-κB and ICAM-1 expression in lungs following intestinal ischemia reperfusion in rats[J];Chinese Journal of Pathophysiology;2010-08
8 WANG De-xuan,YANG Qing,lIN Rui-xia.Pediatric Nephrology Department.WANG Li,XIONG Xi-shan,The Affiliated Yuying Children's Hospital of Wenzhou Medical College,Wenzhou 325027,China;Protection of L-carnitine on Renal Ischemic Reperfusion Injury in Rats[J];Journal of Chinese Microcirculation;2009-02
9 YAO Shu-tong,SANG Hui,WANG Jia-fu,SHANG Zhan-ping,GAO Lin-lin,SI Yan-hong. Dept. of Pathophysiology,Taishan Medical College,Taian 271000,China;Water Extract of Propolis Ameliorates Mesenteric Microcirculation of Rats Subjected to Small Intestinal Ischemia Reperfusion[J];Journal of Chinese Microcirculation;2009-06
10 Zhang Shubo,Li Baoyong,Gao Xiaozeng,et al.Department of anaesthesia,hebei united university afiliated hospital,Tangshan 063000,China;THE PROTECTIVE EFFECTS OF SEVOFLURANE ON LUNG ISCHEMIA REPERFUSION INJURY IN RATS[J];Chinese Journal of Coal Industry Medicine;2011-08
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