Effects of Microfilaments on the Repair of Wounded Endothelial Monolayers
GU HAI-feng,NI Jun,ZHANG Xing-mei. Department of Plastic Surgery,PLA No.85 Hospital,Shanghai 200052,China
Objective To observe the morphological changes of microfilaments and investigate wound closure and cell proliferation after the disruption of microfilaments. Methods An in vitro wound model,an immunofluorescence microscopy and 3 H-TdR incorporation methods were used in this study. Results A specific sequential changes in microfilaments occourred during wound healing. When the microfilaments were disrupted with cytochalasin B,the wound closure and cell migration were greatly delayed. The endothelial cell 3 H-TdR incorporation was also inhibited,showing a time and dose dependent effect. Conclusion These results suggest that the microfiloment systems may be critical to wound healing and the cytoskeletal systems may directly or indirectly participate in the regulation of cell proliferation and wound healing.
【CateGory Index】： R641