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《Journal of Chinese Microcirculation》 2008-02
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ICAM-1 Inhibition Involving Small Intestinal Protection Induced by Ischemic Postconditioning in Rats

YAO Shu-tong,ZHAO Xiu-mei,LIU Xiu-hua.Dept.of Pathophysiology,PLA General Hospital,Beijing 100853,China  
Objective To investigate the mechanisms underlying amelioration of ischemia/reperfusion(I/R) injury of ischemic postconditioning(I-postC) by studying the expression of intercellular adhesion molecule-1(ICAM-1) and myeloperoxidase(MPO) activity in small intestine subjected to Ischemia/Reperfusion.Methods 32 male Wistar rats were randomly divided into sham,I/R,I-postC and ischemic preconditioning(IPC) groups.Model of small intestinal I/R injury was made by clamping super mesenteric artery(SMA) for 45 min followed by 120 min of reperfusion in rats.HE staining was used to observe the pathology of guts of rats.Myeloperoxidase(MPO),superoxide dismutase(SOD) activities and Malondialehyde(MDA) content in small intestine were detected.The expressions of NF-κB P65 and ICAM-1 in small intestine were detected by Western blot analysis.Results I-postC alleviated significantly the pathologic lesion of small intestine induced by I/R.The levels of MDA content and MPO activity were lower and SOD activity was higher in I-postC group than those in I/R group(P0.05).I/R-induced upregulation of NF-κB P65 and ICAM-1 were relieved by I-postC(P0.01 vs.I/R group).Conclusion I-postC protects small intestine from I/R injury through inhibiting the expression of ICAM-1 and the infiltration of PMN.
【Fund】: 国家自然科学基金(30670822);; 国家自然科学基金重大国际合作(30620130111);; 国家重点基础研究发展计划(2007CB512003)资助项目
【CateGory Index】: R364.13
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