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《Journal of Chinese Microcirculation》 2008-02
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Study on Tumor Necrosis Factor-α and Lipopolysaccharides Inducing Interleukin-12 Expression in Mesangial Cells

WU Yuan,JING Li-ming,CHEN Xiao-wen,HE hui-juan.Department of Microbiology and Immunology,Guangdong Medical College;Institute of Nephrology,Affiliated Hospital to Guangdong Medical College,Zhanjiang 524001,China  
Objective To investigate the effect of LPS and TNF-α on interleukin-12(IL-12) expression in mesangial cells.Methods Mesangial cells were cultured in TNF-α and LPS at different doses and time.The protein synthesis of IL-12 in mesangial cells was measured by ELISA.Results Mesangial cells didn't express IL-12 without stimulation.The secrection of IL-12 stimulated by LPS(10 μg/ml)and TNF-α(20 ng/ml) was significantly increased.LPS on dose and time dependant induced the production of IL-12.But the expression of IL-12 decreased with increase of doses and time of LPS stimulant.Conclusion The expression of IL-12 in mesangial cells could be upregulated by LPS and TNF-α,but tolerated to LPS chronic and high doses stimulation.
【CateGory Index】: R392
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