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《Journal of Plant Genetic Resources》 2003-03
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Study on the Transferability of Wheat EST-SSRs

LI Hong-wei 1, 2, LIU Shu-dong 2, GAO Li-feng 1, JIA Ji-zeng 1 ( 1Key lab of Crop Germplasm & Biotechnology, MOA, Institute of Crop Germplasm Resources, CAAS, Beijing 100081; 2 Department of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yang ling 712100)  
EST-SSRs, a new kind of microsatellites (SSRs), was derived from expressed sequence tags (ESTs). A total of 597 EST-SSRs primer pairs were developed in this study, of which about 80%, 65.8%, 62.1% and 58.1% amplified products successfully in wheat, maize, rice and soybean, respectively. A total of 255 primer sets (42.7%) worked across the four species. Sequence similarity alignment of the 255 universal ESTs containing SSRs against database in GenBank indicated that 63 ESTs (30%) were associated with protein destination, and the rest were involved in metabolism, transcription, cellular development, defense system and other pathways related. It is of significance that these EST-SSRs markers will be highly valuable not only for wheat genomics also for comparative genomics among species.
【CateGory Index】: S512.1
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