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《Journal of Plant Genetic Resources》 2003-03
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Study on the Transferability of Wheat EST-SSRs

LI Hong-wei 1, 2, LIU Shu-dong 2, GAO Li-feng 1, JIA Ji-zeng 1 ( 1Key lab of Crop Germplasm & Biotechnology, MOA, Institute of Crop Germplasm Resources, CAAS, Beijing 100081; 2 Department of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yang ling 712100)  
EST-SSRs, a new kind of microsatellites (SSRs), was derived from expressed sequence tags (ESTs). A total of 597 EST-SSRs primer pairs were developed in this study, of which about 80%, 65.8%, 62.1% and 58.1% amplified products successfully in wheat, maize, rice and soybean, respectively. A total of 255 primer sets (42.7%) worked across the four species. Sequence similarity alignment of the 255 universal ESTs containing SSRs against database in GenBank indicated that 63 ESTs (30%) were associated with protein destination, and the rest were involved in metabolism, transcription, cellular development, defense system and other pathways related. It is of significance that these EST-SSRs markers will be highly valuable not only for wheat genomics also for comparative genomics among species.
【CateGory Index】: S512.1
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Chinese Journal Full-text Database 9 Hits
1 ZHENG Yi-qi1,2,LIU Jian-xiu2(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Advances in construction and application of turfgrass molecular genetic maps[J];Acta Prataculturae Sinica;2009-01
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9 CHAI Cheng-sena,JIANG Weia,LI Naa,LIU Chuana,WEN Xue-meia,ZHAO Hui-xiana,HU Sheng-wub(a College of Life Sciences,b College of Agronomy,Northwestern A&F University,Yangling,Shaanxi 712100,China);The transferability of EST-SSR primers and markers between monocotyledonous and dicotyledonous crops[J];Journal of Northwest A & F University(Natural Science Edition);2010-01
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