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Genetic Integrity Analysis of 30 Maize Accessions by SSR

MA Yan-fei1,2,LU Xin-xiong2,CHEN Xiao-ling2,ZHANG Zhi-e2,XIN Ping-ping2,WANG Jian-hua1(1College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094;2Key Laboratory of Crop Germplasm &Biotechnology,Ministry of Agriculture/ Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081)  
The genetic integrity of 30 accessions of maize(Zea mays L.) stored in national genebank of China was investigated by 70 SSR primers.Each accession was represented by two sub-populations regenerated in different year.The genetic similarity between the two sub-populations of the same accession is all above 0.80,except for 0.77 in Hongbaogu(accession number 00230080).Cluster analysis based on the similarity coefficient showed that Er-Huang(accession number 00210055) and HongBaogu diverged from each other,falling into two branches,and that each one cluster was created by two sub-populations of the same accessions,which were multiplied in different years.The reasons for the cluster divergence and feasibility of SSR for genetic integrity analysis in maize were discussed.
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