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《Journal of Plant Genetic Resources》 2015-01
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Isolation and Molecular Characteristics Analysis of Soybean Transcription Factor Gene GmMYB174

WEI Mai-ling;PEI Li-li;LIU Jia-ming;MIN Dong-hong;CHEN Ming;LI Lian-cheng;MA You-zhi;XU Zhao-shi;ZHANG Xiao-hong;College of Life Sciences,Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops,Ministry of Agriculture;  
The full sequence of GmMYB174 was isolated from soybean,including 1086 bp and encoding 361 amino acids and belonged to MYB-related family. Bioinformatics analysis indicated that GmMYB174 shared high similarity with tomato,alfalfa and grape,containing 2 conserved locus and a motif of SHAQKFF. Subcellular localization analysis showed that GmMYB174 located in nucleus. Expression analysis showed that GmMYB174 expressed in many tissues and peaked in epicotyl. Promoter analysis indicated that GmMYB174 contained ABRE,MYB,MYC,LTRE and GT-1. Q-RT-PCR showed that GmMYB174 involved in various abiotic stresses such as drought,salt,and ABA. Therefore,GmMYB174 might participated in multi-environmental stress responses.
【Fund】: 转基因生物新品种培育科技重大专项(2014ZX0800203B);; 陕西省科技计划项目(2014K02-04-05)
【CateGory Index】: S565.1
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