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《Chinese Critical Care Medicine》 2005-06
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Effects of combined naloxone and methylprednisolone on nuclear factor-κB expression in the lung in acute lung injury in rats

ZHANG Qiujin  , SHEN Hong, ZHANG Wei, LI Yinping, LI Tanshi.  EmergencyDepartment, General Hospital of PLA, Beijing 100853, China  
Objective To investigate the effects of a combination of naloxone and methylprednisolone on nuclear factorκB (NFκB) p65 expression in the lung tissue in lipopolysaccharide (LPS) induced acute lung injury(ALI) in rats. Methods ALI models were reproduced by intratracheal instillation of LPS ( 3 mg/kg ). Four hours after LPS instillation, rats were randomly divided into five groups: normal saline group, LPS group, methylprednisolone group, naloxone group (LPS+naloxone) and combined drug group (LPS+naloxone+ methylprednisolone). The level of interleukin8 (IL8) in serum was measured by immunoassay . Meanwhile, the expression of NFκB p65 in the lung tissue was determined with immunohistochemicalstaining. Results Naloxone and methylprednisolone significantly reduced the LPSinducedincrease in IL8 concentrations in serum in vivo, and suppressed the activation of NFκB p65 in the lung tissue. Conclusion NFκB activation is involved in the LPSinduced ALI in rats. Combinationof naloxone and methylprednisolone could suppress the increase of IL8 content and NFκB activation in the lung tissue of rat in vivo in our experiment.
【Fund】: 解放军总后“十五”指令性课题(04LX043)
【CateGory Index】: R563
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