Role of Nitric Oxide in the Protective Effect of Bismuth Potassium Citrate on the Gastric Mucosal Lesion in Rats
LE Qiao-Liang (Department of Gastroenterology,Fuzhou General Hospital,Fuzhou 350025)
Objective:To observe the role of nitric oxide in the protective effect of bismuth potassium citrate on the gastric mucosal lesion in rats.Methods:Before gastric mucosal lesion was made in rats by pure alcohol,bismuth potassium citrate(80 mg/kg)was administered into the gastric chamber and Nω-nitro-L-arginine methyl ester(L-NAME,4 mg/kg),L-arginine(250 mg/kg)or D-arginine(250 mg/kg)were injected into the vein.Gastric mucosal blood flow(GMBF)was assessed with laser Doppler flowmetry(LDF),gastric mucosal and serum NO-_2/NO-_3 were measured by cadmium granulation reduction and colorimetric method,and the changes of ulcer index and the severity of tissues necrosis and neutrophils infiltration were observed.Results:Ulcer index of the bismuth potassium citrate group was much lower than that in control group(P0.01),and the degree of tissues necrosis and neutrophils infiltration were much milder than those of the control group(P0.01). The protective effect of bismuth potassium citrate was significantly decreased by prior administration of L-NAME. The inhibitory effect of L-NAME was antagonized by prior administration of L-arginine,but not D-arginine. Bismuth potassium citrate directly into the gastric chamber obviously increased GMBF,gastric mucosal and serum NO-2/NO-3,this effect was prevented by pretreatment with L-NAME.Conclusion:Nitric oxide should be involved in the mucosal protective of bismuth potassium citrate.
【CateGory Index】： R96