Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Misdiagnostics》 2007-03
Add to Favorite Get Latest Update

Changes of Biochemistry Factors Ca~(2+) ,etc.in Inhibiting the Proliferation of HepG2 Cells with Matrine

ZHANG Jian-min,LI Peng,SI Wei-ke (Department of Laboratory Medical Science,Third Military Medical University, Chongqing 400038)  
Objective: To understand the anti-tumor function of Matrine,we examined the changes of biochemistry factors in HepG2 cells after inhibited by Matrine.Method: Different doses of Matrine treated HepG2, stained the cell with Fluo 3/AM, and checked Ca~ 2+ under the Laser Scanning Confocal Microscope.Changes of AFP,PCNA,cAMP and cGMP were detected with immunocytochemistry and radioimmunoassay;hepatocellulors′ GGT(HSGGT) was isolated with affinity chromatography;enzymatic activation of HSGGT and GGT were detected with enzymatic assay.Result: In treated cells, the concentration of Ca~ 2+ increased; the index of cells growth inhibition -cAMP rose and the ratio of cAMP to cGMP rose; the index of cells proliferation-AFP、PCNA、cGMP、GGT、HSGGT-all fell.Conclusion: Correlated Biochemistry factors change in HepG2 cells after treated by Matrine,which may be relative to the antitumor function of Matrine.
【Fund】: 全军医药卫生科研基金项目(编号:01MA171)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Cai Huaiyang1,Yin Fei1,Du Shaobo1,Zhang Ruimin2 (1 Department of Gastroenterology,the Fourth Hospital of Hebei Medical University. Shijiazhuang 050011;2 Handan Town Center Hospital of Hebei Province,Handan 056001);Different density matrine to liver cancer cell multiplication and JAK-STAT signal transduction influence[J];Pharmacology and Clinics of Chinese Materia Medica;2010-02
【Citations】
Chinese Journal Full-text Database 3 Hits
1 SI Wei ke, CHENG An, LI Peng, LIU Bing, GAO Li hong, YAO Jie (Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038,China);Study on apoptosis of human hepatoma cell line HepG_2 induced by matrine[J];Acta Academiae Medicine Militaris Tertiae;2001-07
2 ZHANG Yan Jun 1,XIA Tian 2,ZHAO Jian Bin 2 1Oncology center, 2Department of Traditional chinese Medicine,Xijing Hospital, Fourth Military Medical University,Xi'an 710033;Effects of Matrine on the differentiation of SMMC 7721 cell lines[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-03
3 Wei Ke Si~1, Jing Pan~2, Hua Lu~2 and Zhao Quan Li~2 1 Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038 China 2 Department of Laboratory Medical Science, Third Military Medical University, Chongqing 400038 China;Study on matrine inhibiting proliferation of HepG2 cell and the relation between its dosage and inhibiting style[J];World Chinese Journal of Digestology;2001-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Xu- fen1* , ZHANG Su- zhan1, ZHENG Shu2, ZHANG Xing2 1.Department of Oncology,the Second Affiliated Hospital School of Medicine, Zhejiang University,Hangzhou 310009,P.R.China 2.Cancer Institute,Zhejiang University, Hangzhou 310009, P.R.China;Effects of Matrine on hTERT- mRNA Expression and Telomerase Activity in K562 Cells[J];Chinese Journal of Cancer;2001-04
2 SI Wei ke*, GAO Li hong, LIU Bing, CHENG An, LI Peng, YAO JieDepartment of Clinical Biochemistry, the Third Military Medical University, Chongqing 400038, P.R.China;Regulation of G_1 Phase Cell Cycle Regulation Factors in Differentiation and Apoptosis of Matrine Induced Hepatoma Cells[J];Chinese Journal of Cancer;2001-08
3 FENG Chun qiong1*, MA Wen li1, ZHENG Wen ling2*1. Institute of Molecular Biology,The First Military Medical University,Guangzhou 510515,P.R.China;2. Institute of Molecular Oncology,Liu Hua Qiao Hospital,Guangzhou 510010,P. R.China);Research Advances on Effect of Arsenic Trioxide on Tumor[J];Chinese Journal of Cancer;2002-12
4 WANG Yong,WU Yu-lian,LIU Ying-bin,TANG Zhe(Department of General Surgery,Second Affiliated Hospital of Zhejiang University,Hangzhou 310009,China);Experimental study on differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by matrine in vitro and its mechanism[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-07
5 ZUO Guo-qing, ZHANG Yan, TANG Wei-xue (1.Department of Gastroenterology, the Second Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400010,China;2.Department of Pathophysiology, Chongqing University of Medical Sciences, Chongqing 400016,China);The expression of apoptosis-associated genes protein in human hepatoma cell lines treated with oxaliplatin[J];Chongqing Medical Journal;2005-01
6 SI Wei ke, CHENG An, LI Peng, LIU Bing, GAO Li hong, YAO Jie (Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038,China);Study on apoptosis of human hepatoma cell line HepG_2 induced by matrine[J];Acta Academiae Medicine Militaris Tertiae;2001-07
7 SI Wei ke, LI Peng, YAO Jie (Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038, China);Effects of matrine on HepG_2 cells at metabolism level and gene level[J];Acta Academiae Medicine Militaris Tertiae;2002-11
8 TANG Zhong zhi, FU Li bo, TANG Ying (Wuhan General Hospital, Guangzhou Command of PLA, Wuhan 430070, China);Inhibition of growth and induction of apoptosis in human hepatoma cell line by tanshinone ⅡA[J];Acta Academiae Medicinae Militaris Tertiae;2003-09
9 DAI Bi tao, JIANG Ji kai, LIU Yu, WANG Li jia, WANG Shi yi (Department of Hematology, College of Pediatrics, Chongqing University of Medical Sciences, Chongqing 400014, China);Effects of matrine on secretion of IL-6 and TNF-α in leukemia cells[J];Acta Academiae Medicinae Militaris Tertiae;2003-22
10 WANG Yuan, SI Wei ke, LI Peng, YAO Jie (Department of Clinical Biochemistry, College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China);Effects of matrine and oxymatrine on the proliferation and the apoptosis of A549 cells[J];Acta Academiae Medicinae Militaris Tertiae;2004-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Lin, AI Zhong li, LIU Zhi su,et al Department of General Surgery of the Second Affiliated Hospital of Hubei Medical University, Wuhan 430071, P R China;An in vitro study of effect of Kushen compound preparation on human colon cancer cell line SW480[J];CHINESE JOURNAL OF CANCER;2000-04
2 LI Xu- fen1* , ZHANG Su- zhan1, ZHENG Shu2, ZHANG Xing2 1.Department of Oncology,the Second Affiliated Hospital School of Medicine, Zhejiang University,Hangzhou 310009,P.R.China 2.Cancer Institute,Zhejiang University, Hangzhou 310009, P.R.China;Effects of Matrine on hTERT- mRNA Expression and Telomerase Activity in K562 Cells[J];Chinese Journal of Cancer;2001-04
3 SI Wei ke*, GAO Li hong, LIU Bing, CHENG An, LI Peng, YAO JieDepartment of Clinical Biochemistry, the Third Military Medical University, Chongqing 400038, P.R.China;Regulation of G_1 Phase Cell Cycle Regulation Factors in Differentiation and Apoptosis of Matrine Induced Hepatoma Cells[J];Chinese Journal of Cancer;2001-08
4 HE Yu juan*,JIANG Ji kai,OU Yi heng,LIU Bei zhong,LIU Xiao shan,ZHANG Yan,MA Lin di,TU Zhi guangDepartment of Clinical Bio chemistry,Chongqing University of Medical Sciences,Chongqing 400016,P.R.China;Matrine Affects Early Expression ofProto-oncogenes in K562 Cells[J];Chinese Journal of Cancer;2002-04
5 LIU Bei zhong1, JIANG Ji kai1, HE Yu juan1, ZHANG Yan1, LIU Xiao shan21. Department of Clinical Biochemistry,Chongqing Medical University,Chongqing 400016,P.R.China;2. Department of Hematology,College of Clinical Medicine,Chongqing Medical University,Chongqing 400016,P.R.China;Effects of Matrine on the Activity of Protein Tyrosine Kinase and Phosphatase in K562 Cells[J];Chinese Journal of Cancer;2002-12
6 Zhu Zhi, Zhu Ming Hua Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, P.R.China;Research Advances on p53 Gene Network[J];Chinese Journal of Cancer;2003-05
7 Wan Xuying,Chen Zhe TCM Section,Changhai Hospital of Second Military Medical University,Shanghai (200433);Primary Liver Cancer Treated by Matrine Injection with TACE Operation[J];Journal of Zhejiang College of Tcm;2002-02
8 HE Song,ZUO Guo-qing,ZHANG Yan,et al.(1.Department of Gastroenterology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;2.Department of Pathophysiology,Chongqing Medical University,Chongqing 400016,China);Effects of matrine on proliferation and telomerase activity in human hepatocellular cell line HepG2[J];Chongqing Medicine;2008-03
9 ZHAN Gang,FENG Lai-yun(Department of General Surgery,161 central Hospital of PLA,Wuhan,Hubei Provinvce 430010,China);Effect of matrine on apoptosis and Bad expression of colorectal cancer cells in vitro[J];Chongqing Medicine;2009-08
10 REN Xin~1,YU Fang~1,YAN Jian-yi~2,ZHAO Dong-li~1 (Medical College of Dalian University,Dalian 116622,China);Analysis of alkaloid content in different organs in Sophora flavescens[J];Journal of Dalian University;2006-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 GUO Qi,SUN Han,LIANG Chong-Yang,ZHANG Shu-Qin,LIU Zhi-Yi,SUN Fei.Institute of Regenerative Medical Science,Jilin University,Changchun 130021;Inhibiting and apoptosis-inducing effects of recombinant Ganoderma Lucidum immunoregulatory protein on HL60 cells[J];Chinese Journal of Immunology;2010-06
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 CAI Hai-ying YE Yan- li LU Sheng-xian (Cancer Institute, Sun Yat-sen University of Medical Sciences.Guangzhou. 5 10060);ANALYSIS OF MALIGNANT TRANS FORMATION CHARACTERISTICS OF RAT ESOPHAGEAL EPITHELIUM USING FLOW CYTOMETRY[J];Chinese Journal of Cancer;1992-04
2 LIANG Yong-ju PAN Qi-chao(Cancer Institute , Sun Yat-Sen University of Medical Sciences Guangzhou·510060);EFFECTS OF MA-TE-LING INJECTION ON CYTOLINETIC[J];CHINESE JOURNAL OF CANCER;1995-06
3 SI Wei ke, XIAO Tao yuan, KANG Ge fei (Department of Clinical Laboratory Medicine, Third Military Medical University, Chongqing 400038, China);Effects of matrine on cell morphology and related factors of hepatocellular carcinoma cell HepG_2 in vitro[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;2000-06
4 ZHANG Yan Jun 1,XIA Tian 2,ZHAO Jian Bin 2 1Oncology center, 2Department of Traditional chinese Medicine,Xijing Hospital, Fourth Military Medical University,Xi'an 710033;Effects of Matrine on the differentiation of SMMC 7721 cell lines[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1998-03
5 Wei Ke Si~1, Jing Pan~2, Hua Lu~2 and Zhao Quan Li~2 1 Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038 China 2 Department of Laboratory Medical Science, Third Military Medical University, Chongqing 400038 China;Study on matrine inhibiting proliferation of HepG2 cell and the relation between its dosage and inhibiting style[J];World Chinese Journal of Digestology;2001-02
6 Xu Jianguo, Ma Junying, Yang Guisheng, Ren Liansheng, Guo Yadong Zhang Hongxiang(Shanxi Institute of Cancer Research, Taiyuan 030013);Differentiation of Human Leukemia Cell (HL-60) Induced by Sophora flavescens Ait. Decoction[J];China Journal of Chinese Materia Medica;1990-10
7 Zhou Xiaozhou,Guo zhen (Hepatopathy Institute,Shen Zhen Hospital of Traditional Chinese Medicine,Shen Zhen 518033);The Expression of P 53 Protein of HepG 2 Cell lines Induced by Anticancer Agent[J];CHINA JOURNAL OF BASIC MEDICINE IN TRADITIONAL CHINESE MEDICINE;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved